Cloning, characterization, and molecular application of a beta-agarase gene from Vibrio sp strain V134
文献类型：期刊论文
| 作者 | Zhang, Wei-wei; Sun, Li; Sun, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
| 刊名 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY
 |
| 出版日期 | 2007 |
| 卷号 | 73期号:9页码:2825-2831 |
| ISSN号 | 0099-2240 |
| 关键词 | Marine Bacterium Lactococcus-lactis Extracellular-agarase Glycosyl Hydrolases Secretion Signals Genomic Dna Purification Expression Classification Sequences |
| 英文摘要 | V134, a marine isolate of the Vibrio genus, was found to produce a new beta-agarase of the GH16 family. The relevant agarase gene agaV was cloned from V134 and conditionally expressed in Escherichia coli. Enzyme activity analysis revealed that the optimum temperature and pH for the purified recombinant agarase were around 40 degrees C and 7.0. AgaV was demonstrated to be useful in two aspects: first, as an agarolytic enzyme, the purified recombinant AgaV could be employed in the recovery of DNA from agarose gels; second, as a secretion protein, AgaV was explored at the genetic level and used as a reporter in the construction of a secretion signal trap which proved to be a simple and efficient molecular tool for the selection of genes encoding secretion proteins from both gram-positive and gram-negative bacteria. |
| 学科主题 | Biotechnology & Applied Microbiology ; Microbiology |
| URL标识 | 查看原文 |
| 公开日期 | 2010-11-18 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/1736]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Sun, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Wei-wei,Sun, Li,Sun, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. Cloning, characterization, and molecular application of a beta-agarase gene from Vibrio sp strain V134[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2007,73(9):2825-2831. |
| APA | Zhang, Wei-wei,Sun, Li,&Sun, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.(2007).Cloning, characterization, and molecular application of a beta-agarase gene from Vibrio sp strain V134.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,73(9),2825-2831. |
| MLA | Zhang, Wei-wei,et al."Cloning, characterization, and molecular application of a beta-agarase gene from Vibrio sp strain V134".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 73.9(2007):2825-2831. |
入库方式： OAI收割
来源：海洋研究所
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