The effects of CpG-C oligodeoxynucleotides on innate immune responses in Eriocheir sinensis (H. Milne-Edwards, 1853)
文献类型：期刊论文
| 作者 | Zhang, Ying; Wang, Lingling; Zhao, Jianmin; Song, Linsheng; Gai, Yunchao; Dong, Chaohua; Qiu, Limei; Song, LS, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
| 刊名 | AQUACULTURE RESEARCH
 |
| 出版日期 | 2010 |
| 卷号 | 41期号:10页码:e656-e666 |
| 关键词 | Chinese Mitten Crab Plasmacytoid Dendritic Cells SaL.o-saL.r L. Reactive Oxygen Antimicrobial Peptides Litopenaeus-vannamei Signal-transduction Molecular-cloning Bacterial-dna White Shrimp |
| ISSN号 | 1355-557X |
| 英文摘要 | CpG oligodeoxynucleotides (ODNs) can stimulate the immune system, and therefore are widely used as a therapeutic vaccination and immune adjuvant in human. In the present study, CpG-C, a combination of A- and B-class ODN, was injected into Chinese mitten crab Eriocheir sinensis at three doses (0.1, 1 and 10 mu g crab-1), and the reactive oxygen species (ROS) levels, activities of total intracellular phenoloxidase (PO) and lysozyme-like activities, the mRNA transcripts of EsproPO, EsCrustin and EsALF were assayed to evaluate its modulating effects on the immune system of crab. The ROS levels in all treated and control groups were significantly increased from 6 to 24 h, except that ROS in 0.1 mu g CpG-C-treated crabs was comparable to that of the blank at 6 h. The PO activity was significantly enhanced and EsproPO transcripts were down-regulated (P < 0.01) at 6 h after the injection of 0.1 mu g CpG-C, with no significant changes in the other dosage treatments. The lysozyme-like activities and EsCrustin transcripts in the CpG-C-treatment groups were significantly higher than those of controls. The mRNA expression of EsALF remained almost constant in all the groups during the treatment. These results collectively suggested that CpG-C could activate the immune responses of E. sinensis, and might be used as a novel immunostimulant for disease control in crabs. |
| 学科主题 | Fisheries |
| URL标识 | 查看原文 |
| 公开日期 | 2010-11-18 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/1742]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Song, LS, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Ying,Wang, Lingling,Zhao, Jianmin,et al. The effects of CpG-C oligodeoxynucleotides on innate immune responses in Eriocheir sinensis (H. Milne-Edwards, 1853)[J]. AQUACULTURE RESEARCH,2010,41(10):e656-e666. |
| APA | Zhang, Ying.,Wang, Lingling.,Zhao, Jianmin.,Song, Linsheng.,Gai, Yunchao.,...&Song, LS, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.(2010).The effects of CpG-C oligodeoxynucleotides on innate immune responses in Eriocheir sinensis (H. Milne-Edwards, 1853).AQUACULTURE RESEARCH,41(10),e656-e666. |
| MLA | Zhang, Ying,et al."The effects of CpG-C oligodeoxynucleotides on innate immune responses in Eriocheir sinensis (H. Milne-Edwards, 1853)".AQUACULTURE RESEARCH 41.10(2010):e656-e666. |
入库方式： OAI收割
来源：海洋研究所
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