Screening of genes expressed in vivo after infection by Vibrio anguillarum M3
文献类型：期刊论文
| 作者 | Zou, Y. -X.; Mo, Z. -L.; Hao, B.; Ye, X. -H.; Guo, D. -S.; Zhang, P. -J.; Mo, ZL, Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
| 刊名 | LETTERS IN APPLIED MICROBIOLOGY
 |
| 出版日期 | 2010 |
| 卷号 | 51期号:5页码:564-569 |
| ISSN号 | 0266-8254 |
| 关键词 | Virulence Identification Vulnificus Cloning |
| 英文摘要 | Aims: Genes uniquely expressed in vivo may contribute to the overall pathogenicity of an organism and are likely to serve as potential targets for the development of new vaccine. This study aims to screen the genes expressed in vivo after Vibrio anguillarum infection by in vivo-induced antigen technology (IVIAT). Methods and Results: The convalescent-phase sera were obtained from turbot (Scophthalmus maximus) survived after infection by the virulent V. anguillarum M3. The pooled sera were thoroughly adsorbed with M3 cells and Escherichia coli BL21 (DE3) cells. A genomic expression library of M3 was constructed and screened for the identification of immunogenic proteins by colony immunoblot analysis with the adsorbed sera. After three rounds of screening, 19 putative in vivo-induced (ivi) genes were obtained. These ivi genes were catalogued into four functional groups: regulator/signalling, metabolism, biological process and hypothetical proteins. Three ivi genes were insertion-mutated, and the growth and 50% lethal dose (LD50) of these mutants were evaluated. Conclusions: The identification of ivi genes in V. anguillarum M3 sheds light on understanding the bacterial pathogenesis and provides novel targets for the development of new vaccines and diagnostic reagents. Significance and Impact of the Study: To the best of our knowledge, this is the first report describing in vivo-expressed genes of V. anguillarum using IVIAT. The screened ivi genes in this study could be new virulent factors and targets for the development of vaccine, which may have implications for the development of diagnostic regents. |
| 学科主题 | Biotechnology & Applied Microbiology ; Microbiology |
| URL标识 | 查看原文 |
| 公开日期 | 2010-11-18 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/1754]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Mo, ZL, Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zou, Y. -X.,Mo, Z. -L.,Hao, B.,et al. Screening of genes expressed in vivo after infection by Vibrio anguillarum M3[J]. LETTERS IN APPLIED MICROBIOLOGY,2010,51(5):564-569. |
| APA | Zou, Y. -X..,Mo, Z. -L..,Hao, B..,Ye, X. -H..,Guo, D. -S..,...&Mo, ZL, Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.(2010).Screening of genes expressed in vivo after infection by Vibrio anguillarum M3.LETTERS IN APPLIED MICROBIOLOGY,51(5),564-569. |
| MLA | Zou, Y. -X.,et al."Screening of genes expressed in vivo after infection by Vibrio anguillarum M3".LETTERS IN APPLIED MICROBIOLOGY 51.5(2010):564-569. |
入库方式： OAI收割
来源：海洋研究所
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