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Molecular cloning, characterization and mRNA expression of peroxiredoxin in Zhikong scallop Chlamys farreri

文献类型：期刊论文

作者Cong, Ming1,2; Ni, Duojiao1,2; Song, Linsheng1; Wang, Lingling1; Zhao, Jianmin1; Qiu, Limei1; Li, Ling1,2
刊名MOLECULAR BIOLOGY REPORTS
出版日期2009-07-01
卷号36期号:6页码:1451-1459
关键词Chlamys Farreri Fluorescent Real-time Quantitative Pcr Immune Response Listonella Anguillarum Peroxiredoxin
ISSN号0301-4851
DOI10.1007/s11033-008-9335-z
文献子类Article
英文摘要Peroxiredoxin V (PRX V) is known as an atypical 2-cysteine peroxiredoxin that protects the organisms against various oxidative stresses and functions in signal transduction. The cDNA of a PRX V gene (designated as CfPRX) was cloned from scallop Chlamys farreri. The full-length sequence of CfPRX cDNA was of 2,179 bp with a 564 bp open reading frame encoding a peptide of 187 amino acids. Sequence comparison showed that CfPRX shared higher identities with PRX Vs than that with other isoforms of PRX, indicating CfPRX was a member of the PRX V family. Fluorescent real-time quantitative PCR analysis revealed the presence of CfPRX transcripts in gill filaments, adductor muscle, heart, gonad, kidney and hemocytes, and the stimulation of Listonella anguillarum significantly (P < 0.01) enhanced the mRNA expression of CfPRX in hemocyte. These results indicated that CfPRX was a constitutive and inducible acute-phase protein which was involved in the immune resistance to L. anguillarum stimulation.; Peroxiredoxin V (PRX V) is known as an atypical 2-cysteine peroxiredoxin that protects the organisms against various oxidative stresses and functions in signal transduction. The cDNA of a PRX V gene (designated as CfPRX) was cloned from scallop Chlamys farreri. The full-length sequence of CfPRX cDNA was of 2,179 bp with a 564 bp open reading frame encoding a peptide of 187 amino acids. Sequence comparison showed that CfPRX shared higher identities with PRX Vs than that with other isoforms of PRX, indicating CfPRX was a member of the PRX V family. Fluorescent real-time quantitative PCR analysis revealed the presence of CfPRX transcripts in gill filaments, adductor muscle, heart, gonad, kidney and hemocytes, and the stimulation of Listonella anguillarum significantly (P < 0.01) enhanced the mRNA expression of CfPRX in hemocyte. These results indicated that CfPRX was a constitutive and inducible acute-phase protein which was involved in the immune resistance to L. anguillarum stimulation.
学科主题Biochemistry & Molecular Biology
URL标识查看原文
语种英语
WOS记录号WOS:000266454500034
公开日期2010-12-22
源URL[http://ir.qdio.ac.cn/handle/337002/2887]  
专题海洋研究所_实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Cong, Ming,Ni, Duojiao,Song, Linsheng,et al. Molecular cloning, characterization and mRNA expression of peroxiredoxin in Zhikong scallop Chlamys farreri[J]. MOLECULAR BIOLOGY REPORTS,2009,36(6):1451-1459.
APA Cong, Ming.,Ni, Duojiao.,Song, Linsheng.,Wang, Lingling.,Zhao, Jianmin.,...&Li, Ling.(2009).Molecular cloning, characterization and mRNA expression of peroxiredoxin in Zhikong scallop Chlamys farreri.MOLECULAR BIOLOGY REPORTS,36(6),1451-1459.
MLA Cong, Ming,et al."Molecular cloning, characterization and mRNA expression of peroxiredoxin in Zhikong scallop Chlamys farreri".MOLECULAR BIOLOGY REPORTS 36.6(2009):1451-1459.

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来源：海洋研究所

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