Comparison of photobioreactors and optimal light regime for rapid vegetative propagation of transgenic Undaria pinnatifida gametophytes
文献类型：期刊论文
| 作者 | Deng, Xiangyuan1,2; Qin, Song1; Zhang, Yichen1,2; Jiang, Peng1 |
| 刊名 | JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2009-10-01 |
| 卷号 | 84期号:10页码:1486-1492 |
| 关键词 | Bubble-column Bioreactor Airlift Bioreactor Vegetative Propagation Undaria Pinnatifida Gametophytes Light Transgenic |
| ISSN号 | 0268-2575 |
| DOI | 10.1002/jctb.2206 |
| 文献子类 | Article |
| 英文摘要 | BACKGROUND: Previously, tachyplesin gene (tac) has been successfully transferred into Undaria pinnatifida gametophytes using the method of microprojectile bombardment transformation. The objectives of this study were to compare and evaluate the performance of bubble-column and airlift bioreactors to determine a preferred configuration of bioreactor for vegetative propagation of transgenic U. pinnatifida gametophytes, and to then investigate the influence of light on vegetative propagation of these gametophytes, including incident light intensity, photoperiod and light quality to resolve the problems of rapid vegetative propagation within the selected bioreactor.; BACKGROUND: Previously, tachyplesin gene (tac) has been successfully transferred into Undaria pinnatifida gametophytes using the method of microprojectile bombardment transformation. The objectives of this study were to compare and evaluate the performance of bubble-column and airlift bioreactors to determine a preferred configuration of bioreactor for vegetative propagation of transgenic U. pinnatifida gametophytes, and to then investigate the influence of light on vegetative propagation of these gametophytes, including incident light intensity, photoperiod and light quality to resolve the problems of rapid vegetative propagation within the selected bioreactor. RESULTS: Experimental results showed that final dry cell density in the airlift bioreactor was 12.7% higher than that in the bubble-column bioreactor under the optimal aeration rate of 1.2 L air min(-1) L-1 culture. And a maximum final dry cell density of 2830 mg L-1 was obtained within the airlift bioreactor using blue light at 40 mu mol m(-2) s(-1) with a light/dark cycle of 14/10 (h). Polymerase chain reaction (PCR) analysis indicated that genes (bar and tac) were not lost during rapid vegetative propagation within the airlift bioreactor. CONCLUSION: The airlift bioreactor was shown to be much more suitable for rapid vegetative propagation of transgenic U. pinnatifida gametophytes than the bubble-column bioreactor in the laboratory. The use of blue light allows improvement of vegetative propagation of transgenic U. pinnatifida gametophytes. (C) 2009 Society of Chemical Industry |
| 学科主题 | Biotechnology & Applied Microbiology ; Chemistry, Multidisciplinary ; Engineering, Chemical |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000270403800009 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/2895]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Deng, Xiangyuan,Qin, Song,Zhang, Yichen,et al. Comparison of photobioreactors and optimal light regime for rapid vegetative propagation of transgenic Undaria pinnatifida gametophytes[J]. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,2009,84(10):1486-1492. |
| APA | Deng, Xiangyuan,Qin, Song,Zhang, Yichen,&Jiang, Peng.(2009).Comparison of photobioreactors and optimal light regime for rapid vegetative propagation of transgenic Undaria pinnatifida gametophytes.JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,84(10),1486-1492. |
| MLA | Deng, Xiangyuan,et al."Comparison of photobioreactors and optimal light regime for rapid vegetative propagation of transgenic Undaria pinnatifida gametophytes".JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 84.10(2009):1486-1492. |
入库方式： OAI收割
来源：海洋研究所
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