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cDNA cloning and gene expression pattern following bacterial challenge of peroxinectin in Chinese shrimp Fenneropenaeus chinensis

文献类型：期刊论文

作者Dong, Bo; Liu, Fengsong; Gao, Hongwei; Wang, Bing; Xiang, Jianhai
刊名MOLECULAR BIOLOGY REPORTS
出版日期2009-11-01
卷号36期号:8页码:2333-2339
关键词Cell Adhesion Gene Peroxinectin Expression Pattern Real-time Rt-pcr Fenneropenaeus Chinensis
ISSN号0301-4851
DOI10.1007/s11033-009-9453-2
文献子类Article
英文摘要Peroxinectin, a cell-adhesive hemoperoxidase that binds superoxide dismutase and mediates blood cells adhesion and migration in invertebrate, is believed to play an important role in cellular immune reaction. In this study, we reported a new peroxinectin gene homologue from Chinese shrimp Fenneropenaeus chinensis. Based on expressed sequence tags (ESTs) of haemocyte cDNA library, we cloned a 2,611 bps full-length cDNA of peroxinectin gene homologue encoded 801 amino acids. Motif scanning of the predicted polypeptide revealed a peroxidase domain and an integrin binding motif (Lys-Gly-Asp, KGD). Peroxinectin gene expressed constitutively in haemocyte as determined by quantitative real-time RT-PCR, the expression level varied following bacterial challenge. These findings suggested that peroxinectin expression is susceptible to exterior stimulus and maintains at a high expression level during bacterial infection.; Peroxinectin, a cell-adhesive hemoperoxidase that binds superoxide dismutase and mediates blood cells adhesion and migration in invertebrate, is believed to play an important role in cellular immune reaction. In this study, we reported a new peroxinectin gene homologue from Chinese shrimp Fenneropenaeus chinensis. Based on expressed sequence tags (ESTs) of haemocyte cDNA library, we cloned a 2,611 bps full-length cDNA of peroxinectin gene homologue encoded 801 amino acids. Motif scanning of the predicted polypeptide revealed a peroxidase domain and an integrin binding motif (Lys-Gly-Asp, KGD). Peroxinectin gene expressed constitutively in haemocyte as determined by quantitative real-time RT-PCR, the expression level varied following bacterial challenge. These findings suggested that peroxinectin expression is susceptible to exterior stimulus and maintains at a high expression level during bacterial infection.
学科主题Biochemistry & Molecular Biology
URL标识查看原文
语种英语
WOS记录号WOS:000270984600039
公开日期2010-12-22
源URL[http://ir.qdio.ac.cn/handle/337002/2899]  
专题海洋研究所_实验海洋生物学重点实验室
作者单位Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714		 Dong, Bo,Liu, Fengsong,Gao, Hongwei,et al. cDNA cloning and gene expression pattern following bacterial challenge of peroxinectin in Chinese shrimp Fenneropenaeus chinensis[J]. MOLECULAR BIOLOGY REPORTS,2009,36(8):2333-2339.
APA Dong, Bo,Liu, Fengsong,Gao, Hongwei,Wang, Bing,&Xiang, Jianhai.(2009).cDNA cloning and gene expression pattern following bacterial challenge of peroxinectin in Chinese shrimp Fenneropenaeus chinensis.MOLECULAR BIOLOGY REPORTS,36(8),2333-2339.
MLA Dong, Bo,et al."cDNA cloning and gene expression pattern following bacterial challenge of peroxinectin in Chinese shrimp Fenneropenaeus chinensis".MOLECULAR BIOLOGY REPORTS 36.8(2009):2333-2339.

入库方式： OAI收割

来源：海洋研究所

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