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Functional biosynthesis of an allophycocyan beta subunit in Escherichia coli

文献类型：期刊论文

作者Ge, Baosheng1,2; Sun, Haixiang3; Feng, Yang1; Yang, Jinying1; Qin, Song4
刊名JOURNAL OF BIOSCIENCE AND BIOENGINEERING
出版日期2009-03-01
卷号107期号:3页码:246-249
关键词Allophycocyanin Beta Subunit Bilin Lyase Biosynthesis Chromophore Escherichia Coli
ISSN号1389-1723
DOI10.1016/j.jbiosc.2008.11.014
文献子类Article
英文摘要Allophycocyanin is a phycobiliprotein with various biological and pharmacological properties. An expression vector was constructed using CpeS as the bilin lyase for the allophycocyanin beta subunit, resulting in overexpression of a fluorescent allophycocyanin beta-subunit in Escherichia coli. A high-density cell culture was developed using a continuous feeding strategy. After 16 h of culture, the dry cell density reached 21.4 g 1(-1), the expression of the allophycocyanin beta-subunit was 0.86 g l(-1) broth, and the relative chromoprotein yield was 81.4%. The recombinant protein showed spectral features similar to native allophycocyanin, which provide an efficient methodology for large-scale production of this valuable fluorescent protein. (C) 2008, The Society for Biotechnology, Japan. All rights reserved.; Allophycocyanin is a phycobiliprotein with various biological and pharmacological properties. An expression vector was constructed using CpeS as the bilin lyase for the allophycocyanin beta subunit, resulting in overexpression of a fluorescent allophycocyanin beta-subunit in Escherichia coli. A high-density cell culture was developed using a continuous feeding strategy. After 16 h of culture, the dry cell density reached 21.4 g 1(-1), the expression of the allophycocyanin beta-subunit was 0.86 g l(-1) broth, and the relative chromoprotein yield was 81.4%. The recombinant protein showed spectral features similar to native allophycocyanin, which provide an efficient methodology for large-scale production of this valuable fluorescent protein. (C) 2008, The Society for Biotechnology, Japan. All rights reserved.
学科主题Biotechnology & Applied Microbiology ; Food Science & Technology
URL标识查看原文
语种英语
WOS记录号WOS:000265311200006
公开日期2010-12-22
源URL[http://ir.qdio.ac.cn/handle/337002/2913]  
专题海洋研究所_实验海洋生物学重点实验室
作者单位1.China Univ Petr, Ctr Biotechnol & Bioengn, Qingdao 266555, Shandong, Peoples R China
2.Huaihai Inst Technol, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Jiangsu, Peoples R China
3.China Univ Petr, Coll Chem & Chem Engn, Dongying 257061, Shandong, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Ge, Baosheng,Sun, Haixiang,Feng, Yang,et al. Functional biosynthesis of an allophycocyan beta subunit in Escherichia coli[J]. JOURNAL OF BIOSCIENCE AND BIOENGINEERING,2009,107(3):246-249.
APA Ge, Baosheng,Sun, Haixiang,Feng, Yang,Yang, Jinying,&Qin, Song.(2009).Functional biosynthesis of an allophycocyan beta subunit in Escherichia coli.JOURNAL OF BIOSCIENCE AND BIOENGINEERING,107(3),246-249.
MLA Ge, Baosheng,et al."Functional biosynthesis of an allophycocyan beta subunit in Escherichia coli".JOURNAL OF BIOSCIENCE AND BIOENGINEERING 107.3(2009):246-249.

入库方式： OAI收割

来源：海洋研究所

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