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Construction and characterization of a fosmid library for pathogenic bacterium Vibrio anguillarum

文献类型：期刊论文

作者Han Yifan2; Mo Zhaolan1; Mao Yunxiang2; Xiao Peng2; Li Jie1; Hao Bin2; Guo Dongsheng2
刊名CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
出版日期2009-08-01
卷号27期号:3页码:519-526
关键词End-sequencing Fosmid Library Pcr Screening Vibrio Anguillarum
ISSN号0254-4059
DOI10.1007/s00343-009-9230-z
文献子类Article
英文摘要Vibrio anguillarum is a common bacterial pathogen in fish. However, little is known about its pathogenic mechanism, in part, because the entire genome has not been completely sequenced. We constructed a fosmid library for V. anguillarum containing 960 clones with an average insert size of 37.7 kb and 8.6-fold genome coverage. We characterized the library by end-sequencing 50 randomly selected clones. This generated 93 sequences with a total length of 57 485 by covering 1.4% of the whole genome. Of these sequences, 58 (62.4%) were homologous to known genes, 30 (32.3%) were genes with hypothetical functions, and the remaining 5 (5.3%) were unknown genes. We demonstrated the utility of this library by PCR screening of 10 genes. This resulted in an average of 6.2 fosmid clones per screening. This fosmid library offers a new tool for gene screening and cloning of V. anguillarum, and for comparative genomic studies among Vibrio species.; Vibrio anguillarum is a common bacterial pathogen in fish. However, little is known about its pathogenic mechanism, in part, because the entire genome has not been completely sequenced. We constructed a fosmid library for V. anguillarum containing 960 clones with an average insert size of 37.7 kb and 8.6-fold genome coverage. We characterized the library by end-sequencing 50 randomly selected clones. This generated 93 sequences with a total length of 57 485 by covering 1.4% of the whole genome. Of these sequences, 58 (62.4%) were homologous to known genes, 30 (32.3%) were genes with hypothetical functions, and the remaining 5 (5.3%) were unknown genes. We demonstrated the utility of this library by PCR screening of 10 genes. This resulted in an average of 6.2 fosmid clones per screening. This fosmid library offers a new tool for gene screening and cloning of V. anguillarum, and for comparative genomic studies among Vibrio species.
学科主题Limnology ; Oceanography
URL标识查看原文
语种英语
WOS记录号WOS:000270742000013
公开日期2010-12-22
源URL[http://ir.qdio.ac.cn/handle/337002/2915]  
专题海洋研究所_实验海洋生物学重点实验室
海洋研究所_海洋生物分类与系统演化实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Ocean Univ China, Qingdao 266003, Peoples R China
推荐引用方式
GB/T 7714		 Han Yifan,Mo Zhaolan,Mao Yunxiang,et al. Construction and characterization of a fosmid library for pathogenic bacterium Vibrio anguillarum[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2009,27(3):519-526.
APA Han Yifan.,Mo Zhaolan.,Mao Yunxiang.,Xiao Peng.,Li Jie.,...&Guo Dongsheng.(2009).Construction and characterization of a fosmid library for pathogenic bacterium Vibrio anguillarum.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,27(3),519-526.
MLA Han Yifan,et al."Construction and characterization of a fosmid library for pathogenic bacterium Vibrio anguillarum".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 27.3(2009):519-526.

入库方式： OAI收割

来源：海洋研究所

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