Molecular analysis of the copper-responsive CopRSCD of a pathogenic Pseudomonas fluorescens strain
文献类型：期刊论文
| 作者 | Hu, Yong-hua1,2; Wang, Hua-lei3; Zhang, Min1,2; Sun, Li1 |
| 刊名 | JOURNAL OF MICROBIOLOGY
 |
| 出版日期 | 2009-06-01 |
| 卷号 | 47期号:3页码:277-286 |
| 关键词 | Copabcd Copper Resistance Copr Pseudomonas Fluorescens Virulence |
| ISSN号 | 1225-8873 |
| DOI | 10.1007/s12275-008-0278-9 |
| 文献子类 | Article |
| 英文摘要 | CopRS/CopABCD is one of the known systems that control copper homeostasis in bacteria. Although CopRS/CopABCD homologues are found to exist in Pseudomonas fluorescens, the potential role of this system in P. fluorescens has not been investigated. In this study a genetic cluster, consisting of copR, S, C, and D but lacking copAB, was identified in a pathogenic P. fluorescens strain (TSS) isolated from diseased fish. The copRSCD cluster was demonstrated to be required for full copper resistance and regulated at the transcription level by Cu. Expression of copCD is regulated directly by the two-component response regulator CopR, which also regulates its own expression. Interruption of the regulated expression of copR affected bacterial growth, biofilm formation, and tissue dissemination and survival. A mutant CopR, which lacks the N-terminal signal receiver domain and is constitutively active, was found to have an attenuating effect on bacterial virulence when expressed in TSS. To our knowledge, this is the first report that suggests a link between CopR and bacterial pathogenicity in P. fluorescens.; CopRS/CopABCD is one of the known systems that control copper homeostasis in bacteria. Although CopRS/CopABCD homologues are found to exist in Pseudomonas fluorescens, the potential role of this system in P. fluorescens has not been investigated. In this study a genetic cluster, consisting of copR, S, C, and D but lacking copAB, was identified in a pathogenic P. fluorescens strain (TSS) isolated from diseased fish. The copRSCD cluster was demonstrated to be required for full copper resistance and regulated at the transcription level by Cu. Expression of copCD is regulated directly by the two-component response regulator CopR, which also regulates its own expression. Interruption of the regulated expression of copR affected bacterial growth, biofilm formation, and tissue dissemination and survival. A mutant CopR, which lacks the N-terminal signal receiver domain and is constitutively active, was found to have an attenuating effect on bacterial virulence when expressed in TSS. To our knowledge, this is the first report that suggests a link between CopR and bacterial pathogenicity in P. fluorescens. |
| 学科主题 | Microbiology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000267396900008 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/2921]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Hang Zhou EnzySource Biotechnol Co Ltd, Hangzhou 310030, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Yong-hua,Wang, Hua-lei,Zhang, Min,et al. Molecular analysis of the copper-responsive CopRSCD of a pathogenic Pseudomonas fluorescens strain[J]. JOURNAL OF MICROBIOLOGY,2009,47(3):277-286. |
| APA | Hu, Yong-hua,Wang, Hua-lei,Zhang, Min,&Sun, Li.(2009).Molecular analysis of the copper-responsive CopRSCD of a pathogenic Pseudomonas fluorescens strain.JOURNAL OF MICROBIOLOGY,47(3),277-286. |
| MLA | Hu, Yong-hua,et al."Molecular analysis of the copper-responsive CopRSCD of a pathogenic Pseudomonas fluorescens strain".JOURNAL OF MICROBIOLOGY 47.3(2009):277-286. |
入库方式： OAI收割
来源：海洋研究所
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