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Using the ribosomal internal transcribed spacer (ITS) as a complement marker for species identification of red macroalgae

文献类型：期刊论文

作者Hu, ZiMin1,2; Guiry, Michael D.2; Duan, DeLin1
刊名HYDROBIOLOGIA
出版日期2009-11-01
卷号635期号:1页码:279-287
关键词Complementary Marker Internal Transcribed Spacer Red Macroalgae Species Identification
ISSN号0018-8158
DOI10.1007/s10750-009-9920-8
文献子类Article
英文摘要Barcodes based on mitochondrial cytochrome oxidase (mtDNA CO1) sequences are being used for broad taxonomic groups of animals with demonstrated success in species identification and cryptic species discovery, but it has become clear that complementation by a nuclear marker system is necessary, in particular for the barcoding of plants. Here, we propose the nuclear internal transcribed spacer (ITS) as a potentially usable and complementary marker for species identification of red macroalgae, as well as present a primary workflow for species barcoding. Data show that for most red macroalgal genera (except members of the family Delesseriaceae), the size of ITS region ranges from 600 to 1200 bp, and contains enough variation to generate unique identifiers at either the species or genus levels. Consistent with previous studies, we found that the ITS sequence can resolve closely related species with the same fidelity as mtDNA CO1. Significantly, we confirmed that length polymorphism in the ITS region (including 5.8S rRNA gene) can be utilized as a character to discriminate red macroalgal species. As a complementary marker, the verifiable nuclear ITS region can speed routine identification and the detection of species, advance ecological and taxonomic inquiry, and permit rapid and accurate analysis of red macroalgae.; Barcodes based on mitochondrial cytochrome oxidase (mtDNA CO1) sequences are being used for broad taxonomic groups of animals with demonstrated success in species identification and cryptic species discovery, but it has become clear that complementation by a nuclear marker system is necessary, in particular for the barcoding of plants. Here, we propose the nuclear internal transcribed spacer (ITS) as a potentially usable and complementary marker for species identification of red macroalgae, as well as present a primary workflow for species barcoding. Data show that for most red macroalgal genera (except members of the family Delesseriaceae), the size of ITS region ranges from 600 to 1200 bp, and contains enough variation to generate unique identifiers at either the species or genus levels. Consistent with previous studies, we found that the ITS sequence can resolve closely related species with the same fidelity as mtDNA CO1. Significantly, we confirmed that length polymorphism in the ITS region (including 5.8S rRNA gene) can be utilized as a character to discriminate red macroalgal species. As a complementary marker, the verifiable nuclear ITS region can speed routine identification and the detection of species, advance ecological and taxonomic inquiry, and permit rapid and accurate analysis of red macroalgae.
学科主题Marine & Freshwater Biology
URL标识查看原文
语种英语
WOS记录号WOS:000270652200025
公开日期2010-12-22
源URL[http://ir.qdio.ac.cn/handle/337002/2923]  
专题海洋研究所_实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Natl Univ Ireland, Martin Ryan Inst, Galway, Ireland
推荐引用方式
GB/T 7714		 Hu, ZiMin,Guiry, Michael D.,Duan, DeLin. Using the ribosomal internal transcribed spacer (ITS) as a complement marker for species identification of red macroalgae[J]. HYDROBIOLOGIA,2009,635(1):279-287.
APA Hu, ZiMin,Guiry, Michael D.,&Duan, DeLin.(2009).Using the ribosomal internal transcribed spacer (ITS) as a complement marker for species identification of red macroalgae.HYDROBIOLOGIA,635(1),279-287.
MLA Hu, ZiMin,et al."Using the ribosomal internal transcribed spacer (ITS) as a complement marker for species identification of red macroalgae".HYDROBIOLOGIA 635.1(2009):279-287.

入库方式： OAI收割

来源：海洋研究所

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