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Extra- and intra-cellular ice formation of red seabream (Pagrus major) embryos at different cooling rates

文献类型：期刊论文

作者Li, J.1; Zhang, L. L.1,2; Liu, Q. H.1,2; Xu, X. Z.1,2; Xiao, Z. Z.1; Ma, D. Y.1; Xu, S. H.1; Xue, Q. Z.3
刊名CRYOBIOLOGY
出版日期2009-08-01
卷号59期号:1页码:48-53
关键词Red Seabream Embryo Extra- And Intra-cellular Ice Formation Cooling Rate
ISSN号0011-2240
DOI10.1016/j.cryobiol.2009.04.004
文献子类Article
英文摘要The ice crystal formation is assumed as the most lethal factor for the failure of fish embryo cryopreservation and intracellular ice formation (IIF) plays a central role in cell injury during cooling. The objectives were to observe the morphological changes of red seabream (Pagrus major) embryo during the cooling-thawing process, and to examine the effect of cryoprotectant and cooling rate on the temperatures of oil globule ice formation (T-OIF), extra-cellular ice formation (T-EIF) and intracellular ice formation (T-IIF) using cryomicroscope. After thawing, the morphological changes of embryos were observed and recorded by the video attachment and monitor under the microscope. During the cooling process, three representative phenomena were observed: oil globule gradually turned bright firstly, then the whole field of view flashed and the embryo blackened. Cooling rate affect the temperature of both extra- and intra-cellular ice formations. T-EIF and T-IIF at high cooling rate were much lower than that at low cooling rate. And the value of T-EIF - T-IIF increased from 0.45 to 11.11 degrees C with the increase of cooling rate from 3 to 130 degrees C/min. Taken together, our results suggested that high cooling rate with proper cryoprotectant would be a good option for red seabream embryo cryopreservation. (C) 2009 Elsevier Inc. All rights reserved.; The ice crystal formation is assumed as the most lethal factor for the failure of fish embryo cryopreservation and intracellular ice formation (IIF) plays a central role in cell injury during cooling. The objectives were to observe the morphological changes of red seabream (Pagrus major) embryo during the cooling-thawing process, and to examine the effect of cryoprotectant and cooling rate on the temperatures of oil globule ice formation (T-OIF), extra-cellular ice formation (T-EIF) and intracellular ice formation (T-IIF) using cryomicroscope. After thawing, the morphological changes of embryos were observed and recorded by the video attachment and monitor under the microscope. During the cooling process, three representative phenomena were observed: oil globule gradually turned bright firstly, then the whole field of view flashed and the embryo blackened. Cooling rate affect the temperature of both extra- and intra-cellular ice formations. T-EIF and T-IIF at high cooling rate were much lower than that at low cooling rate. And the value of T-EIF - T-IIF increased from 0.45 to 11.11 degrees C with the increase of cooling rate from 3 to 130 degrees C/min. Taken together, our results suggested that high cooling rate with proper cryoprotectant would be a good option for red seabream embryo cryopreservation. (C) 2009 Elsevier Inc. All rights reserved.
学科主题Biology ; Physiology
URL标识查看原文
语种英语
WOS记录号WOS:000268059000008
公开日期2010-12-22
源URL[http://ir.qdio.ac.cn/handle/337002/2951]  
专题海洋研究所_实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100031, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714		 Li, J.,Zhang, L. L.,Liu, Q. H.,et al. Extra- and intra-cellular ice formation of red seabream (Pagrus major) embryos at different cooling rates[J]. CRYOBIOLOGY,2009,59(1):48-53.
APA Li, J..,Zhang, L. L..,Liu, Q. H..,Xu, X. Z..,Xiao, Z. Z..,...&Xue, Q. Z..(2009).Extra- and intra-cellular ice formation of red seabream (Pagrus major) embryos at different cooling rates.CRYOBIOLOGY,59(1),48-53.
MLA Li, J.,et al."Extra- and intra-cellular ice formation of red seabream (Pagrus major) embryos at different cooling rates".CRYOBIOLOGY 59.1(2009):48-53.

入库方式： OAI收割

来源：海洋研究所

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