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Identification of a novel relish homolog in Chinese shrimp Fenneropenaeus chinensis and its function in regulating the transcription of antimicrobial peptides

文献类型：期刊论文

作者Li, Fuhua1; Yan, Hui1,2; Wang, Dongdong1,2; Priya, T. A. Jose1,2; Li, Shihao1,2; Wang, Bing1; Zhang, Jiquan1; Xiang, Jianhai1
刊名DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
出版日期2009-10-01
卷号33期号:10页码:1093-1101
关键词Relish Immunity Antimicrobial Peptides Transcription Rna Interference Fenneropenaeus Chinensis
ISSN号0145-305X
DOI10.1016/j.dci.2009.06.001
文献子类Article
英文摘要Penaeid shrimp, as an invertebrate, relies on the innate immunity to oppose the microbial invaders. Antimicrobial peptides (AMP) are an integral component of the innate immune system in most organisms and function as an early first line of defense against pathogens, but the knowledge about the pathways to regulate the shrimp AMP gene expression is still absent up to date. In the current study, a Relish homolog (FcRelish) was cloned from Chinese shrimp Fenneropenaeus chinensis. The full length cDNA of FcRelish consists of 2157 bp, including 1512 bp open reading frame, encoding 504 amino acids. The predicted molecular weight of FcRelish is 57 kDa, and the theoretical PI is 7.00. Spatial expression profiles showed that FcRelish had the highest expression levels in the hemocytes and lymphoid organ. Both Vibrio anguillarium and Micrococcus lysodeikticus stimulation to shrimp can affect the transcription profile of FcRelish. Silencing of FcRelish through DsRNA interference can greatly change the transcription profile of AMP. Therefore, we suggest that FcRelish identified in the present study is closely related to the transcription of AMP, and then we inferred that Imd pathway might exist in shrimp. (C) 2009 Elsevier Ltd. All rights reserved.; Penaeid shrimp, as an invertebrate, relies on the innate immunity to oppose the microbial invaders. Antimicrobial peptides (AMP) are an integral component of the innate immune system in most organisms and function as an early first line of defense against pathogens, but the knowledge about the pathways to regulate the shrimp AMP gene expression is still absent up to date. In the current study, a Relish homolog (FcRelish) was cloned from Chinese shrimp Fenneropenaeus chinensis. The full length cDNA of FcRelish consists of 2157 bp, including 1512 bp open reading frame, encoding 504 amino acids. The predicted molecular weight of FcRelish is 57 kDa, and the theoretical PI is 7.00. Spatial expression profiles showed that FcRelish had the highest expression levels in the hemocytes and lymphoid organ. Both Vibrio anguillarium and Micrococcus lysodeikticus stimulation to shrimp can affect the transcription profile of FcRelish. Silencing of FcRelish through DsRNA interference can greatly change the transcription profile of AMP. Therefore, we suggest that FcRelish identified in the present study is closely related to the transcription of AMP, and then we inferred that Imd pathway might exist in shrimp. (C) 2009 Elsevier Ltd. All rights reserved.
学科主题Immunology ; Zoology
URL标识查看原文
语种英语
WOS记录号WOS:000268952800007
公开日期2010-12-22
源URL[http://ir.qdio.ac.cn/handle/337002/2955]  
专题海洋研究所_实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714		 Li, Fuhua,Yan, Hui,Wang, Dongdong,et al. Identification of a novel relish homolog in Chinese shrimp Fenneropenaeus chinensis and its function in regulating the transcription of antimicrobial peptides[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2009,33(10):1093-1101.
APA Li, Fuhua.,Yan, Hui.,Wang, Dongdong.,Priya, T. A. Jose.,Li, Shihao.,...&Xiang, Jianhai.(2009).Identification of a novel relish homolog in Chinese shrimp Fenneropenaeus chinensis and its function in regulating the transcription of antimicrobial peptides.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,33(10),1093-1101.
MLA Li, Fuhua,et al."Identification of a novel relish homolog in Chinese shrimp Fenneropenaeus chinensis and its function in regulating the transcription of antimicrobial peptides".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 33.10(2009):1093-1101.

入库方式： OAI收割

来源：海洋研究所

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