A serpin from Chinese shrimp Fenneropenaeus chinensis is responsive to bacteria and WSSV challenge
文献类型:期刊论文
作者 | Liu, Yichen1,2; Li, Fuhua1; Wang, Bing1; Dong, Bo1; Zhang, Xiaojun1; Xiang, Jianhai1 |
刊名 | FISH & SHELLFISH IMMUNOLOGY |
出版日期 | 2009-03-01 |
卷号 | 26期号:3页码:345-351 |
ISSN号 | 1050-4648 |
关键词 | Serpin Cloning Expression Profile Challenge Fenneropenaeus Chinensis |
DOI | 10.1016/j.fsi.2008.08.016 |
文献子类 | Article |
英文摘要 | Arthropod defence responses (e.g. prophenoloxidase (proPO) activation and Toll pathway initiation) are mediated by serine proteinase cascades and regulated by serpins in haemolymph. A serpin (Fc-serpin) cDNA was cloned from the haemocytes of Fenneropenaeus chinensis by rapid amplification of cDNA ends (RACE) PCR and haemocyte cDNA library screening. The full-length cDNA consists of 1734 bp, encoding 411 amino acids with a calculated molecular mass of 46.55 kDa and a theoretical isoelectric point of 7.70. Fc-serpin contains a typical serpin-like homologue (serine proteinase inhibitors domain). The deduced protein contains a putative signal peptide of 19 amino acids and the serpin's signature sequence ((379)FHCNRPFLFLI(389)). Fc-serpin showed some identity with Pacifastacus leniusculus serpin (42%) and Manduca sexta serpin-6 (34%). The reactive centre loop (RCL) sequences of Fc-serpin, P leniusculus serpin, M. sexta serpin-6 and Bombyx mori serpin-2 are highly similar. An Arg at the PI position of the reactive site indicates that Fc-serpin may have inhibitory activity against prophenoloxidase activating proteinase (PAP) and clotting enzyme. Transcripts of Fc-serpin mRNA were mainly detected in haemocytes and the lymphoid organ by RT-PCR. The variation of the mRNA transcription level in haemocytes followed by artificial infection with bacteria OF white spot syndrome virus (WSSV) was quantified by SYBR Green real-time PCR analysis. Expression profiles of Fc-serpin greatly fluctuated after challenge. This work represents the first report Of a serpin in penaeid shrimp. The data provide clues that Fc-serpin might play potential roles in the innate immunity of shrimp. (C) 2008 Elsevier Ltd. All rights reserved.; Arthropod defence responses (e.g. prophenoloxidase (proPO) activation and Toll pathway initiation) are mediated by serine proteinase cascades and regulated by serpins in haemolymph. A serpin (Fc-serpin) cDNA was cloned from the haemocytes of Fenneropenaeus chinensis by rapid amplification of cDNA ends (RACE) PCR and haemocyte cDNA library screening. The full-length cDNA consists of 1734 bp, encoding 411 amino acids with a calculated molecular mass of 46.55 kDa and a theoretical isoelectric point of 7.70. Fc-serpin contains a typical serpin-like homologue (serine proteinase inhibitors domain). The deduced protein contains a putative signal peptide of 19 amino acids and the serpin's signature sequence ((FHCNRPFLFLI389)-F-379). Fc-serpin showed some identity with Pacifastacus leniusculus serpin (42%) and Manduca sexta serpin-6 (34%). The reactive centre loop (RCL) sequences of Fc-serpin, P leniusculus serpin, M. sexta serpin-6 and Bombyx mori serpin-2 are highly similar. An Arg at the PI position of the reactive site indicates that Fc-serpin may have inhibitory activity against prophenoloxidase activating proteinase (PAP) and clotting enzyme. Transcripts of Fc-serpin mRNA were mainly detected in haemocytes and the lymphoid organ by RT-PCR. The variation of the mRNA transcription level in haemocytes followed by artificial infection with bacteria OF white spot syndrome virus (WSSV) was quantified by SYBR Green real-time PCR analysis. Expression profiles of Fc-serpin greatly fluctuated after challenge. This work represents the first report Of a serpin in penaeid shrimp. The data provide clues that Fc-serpin might play potential roles in the innate immunity of shrimp. (C) 2008 Elsevier Ltd. All rights reserved. |
学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
URL标识 | 查看原文 |
语种 | 英语 |
WOS记录号 | WOS:000265518300001 |
公开日期 | 2010-12-22 |
源URL | [http://ir.qdio.ac.cn/handle/337002/2971] |
专题 | 海洋研究所_实验海洋生物学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Tianjin Normal Univ, Coll Chem & Life Sci, Tianjin 300387, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Yichen,Li, Fuhua,Wang, Bing,et al. A serpin from Chinese shrimp Fenneropenaeus chinensis is responsive to bacteria and WSSV challenge[J]. FISH & SHELLFISH IMMUNOLOGY,2009,26(3):345-351. |
APA | Liu, Yichen,Li, Fuhua,Wang, Bing,Dong, Bo,Zhang, Xiaojun,&Xiang, Jianhai.(2009).A serpin from Chinese shrimp Fenneropenaeus chinensis is responsive to bacteria and WSSV challenge.FISH & SHELLFISH IMMUNOLOGY,26(3),345-351. |
MLA | Liu, Yichen,et al."A serpin from Chinese shrimp Fenneropenaeus chinensis is responsive to bacteria and WSSV challenge".FISH & SHELLFISH IMMUNOLOGY 26.3(2009):345-351. |
入库方式: OAI收割
来源:海洋研究所
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