Enrichment of the hydrogen-producing microbial community from marine intertidal sludge by different pretreatment methods
文献类型：期刊论文
| 作者 | Liu, Hongyan1,2,3; Wang, Guangce1,2; Zhu, Daling2; Pan, Guanghua2 |
| 刊名 | INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
 |
| 出版日期 | 2009-12-01 |
| 卷号 | 34期号:24页码:9696-9701 |
| 关键词 | Pretreatment Hydrogen Production Denaturing Gradient Gel Electrophoresis (Dgge) Microbial Community |
| ISSN号 | 0360-3199 |
| DOI | 10.1016/j.ijhydene.2009.10.025 |
| 文献子类 | Article |
| 英文摘要 | To determine the effects of pretreatment on hydrogen production and the hydrogen-producing microbial community, we treated the sludge from the intertidal zone of a bathing beach in Tianjin with four different pretreatment methods, including acid treatment, heat-shock, base treatment as well as freezing and thawing. The results showed that acid pretreatment significantly promoted the hydrogen production by sludge and provided the highest efficiency of hydrogen production among the four methods. The efficiency of the hydrogen production of the acid-pretreated sludge was 0.86 +/- 0.07 mol H(2)/mol glucose (mean +/- S.E.), whereas that of the sludge treated with heat-shock, freezing and thawing, base method and control was 0.41 +/- 0.03 mol H(2)/mol glucose, 0.17 +/- 0.01 mol H(2)/mol glucose, 0.11 +/- 0.01 mol H(2)/mol glucose and 0.20 +/- 0.04 mol H(2)/mol glucose, respectively. The result of denaturing gradient gel electrophoresis (DGGE) showed that pretreatment methods altered the composition of the microbial community that accounts for hydrogen production. Acid and heat pretreatments were favorable to enrich the dominant hydrogen-producing bacterium, i.e. Clostridium sp., Enterococcus sp. and Bacillus sp., However, besides hydrogen-producing bacteria, much non-hydrogen-producing Lactobacillus sp. was also found in the sludge pretreated with base, freezing and thawing methods. Therefore, based on our results, we concluded that, among the four pretreatment methods using acid, heat-shock, base or freezing and thawing, acid pretreatment was the most effective method for promoting hydrogen production of microbial community. (C) 2009 Professor T. Nejat Veziroglu. Published by Elsevier Ltd. All rights reserved.; To determine the effects of pretreatment on hydrogen production and the hydrogen-producing microbial community, we treated the sludge from the intertidal zone of a bathing beach in Tianjin with four different pretreatment methods, including acid treatment, heat-shock, base treatment as well as freezing and thawing. The results showed that acid pretreatment significantly promoted the hydrogen production by sludge and provided the highest efficiency of hydrogen production among the four methods. The efficiency of the hydrogen production of the acid-pretreated sludge was 0.86 +/- 0.07 mol H-2/mol glucose (mean +/- S.E.), whereas that of the sludge treated with heat-shock, freezing and thawing, base method and control was 0.41 +/- 0.03 mol H-2/mol glucose, 0.17 +/- 0.01 mol H-2/mol glucose, 0.11 +/- 0.01 mol H-2/mol glucose and 0.20 +/- 0.04 mol H-2/mol glucose, respectively. The result of denaturing gradient gel electrophoresis (DGGE) showed that pretreatment methods altered the composition of the microbial community that accounts for hydrogen production. Acid and heat pretreatments were favorable to enrich the dominant hydrogen-producing bacterium, i.e. Clostridium sp., Enterococcus sp. and Bacillus sp., However, besides hydrogen-producing bacteria, much non-hydrogen-producing Lactobacillus sp. was also found in the sludge pretreated with base, freezing and thawing methods. Therefore, based on our results, we concluded that, among the four pretreatment methods using acid, heat-shock, base or freezing and thawing, acid pretreatment was the most effective method for promoting hydrogen production of microbial community. (C) 2009 Professor T. Nejat Veziroglu. Published by Elsevier Ltd. All rights reserved. |
| 学科主题 | Chemistry, Physical ; Energy & Fuels ; Environmental Sciences ; Physics, Atomic, Molecular & Chemical |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000272856400007 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/2975]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China 2.Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Hongyan,Wang, Guangce,Zhu, Daling,et al. Enrichment of the hydrogen-producing microbial community from marine intertidal sludge by different pretreatment methods[J]. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,2009,34(24):9696-9701. |
| APA | Liu, Hongyan,Wang, Guangce,Zhu, Daling,&Pan, Guanghua.(2009).Enrichment of the hydrogen-producing microbial community from marine intertidal sludge by different pretreatment methods.INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,34(24),9696-9701. |
| MLA | Liu, Hongyan,et al."Enrichment of the hydrogen-producing microbial community from marine intertidal sludge by different pretreatment methods".INTERNATIONAL JOURNAL OF HYDROGEN ENERGY 34.24(2009):9696-9701. |
入库方式： OAI收割
来源：海洋研究所
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