A thioredoxin with antioxidant activity identified from Eriocheir sinensis
文献类型：期刊论文
| 作者 | Mu, Changkao1,2; Zhao, Jianmin1; Wang, Lingling1; Song, Linsheng1; Song, Xiaoyan3; Zhang, Huan1,2; Qiu, Limei1; Gai, Yunchao1,2; Cui, Zhaoxia1 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2009-05-01 |
| 卷号 | 26期号:5页码:716-723 |
| 关键词 | Thioredoxin Eriocheir Sinensis Gene Cloning Innate Immunity Mrna Expression Antioxidant Capacity |
| ISSN号 | 1050-4648 |
| DOI | 10.1016/j.fsi.2009.02.024 |
| 文献子类 | Article |
| 英文摘要 | Thioredoxin, with a redox-active disulfide/dithiol in the active site, is the major ubiquitous disulfide reductase responsible for maintaining proteins in their reduced state. In the present study, the cDNA encoding thioredoxin-1 (designated EsTrx1) was cloned from Chinese mitten crab Eriocheir sinensis by using rapid amplification of cDNA ends (RACE) approaches. The full-length cDNA of EsTrx1 was of 641 bp, containing a 51 untranslated region (UTR) of 17 bp, a 3' UTR of 306 bp with a poly (A) tail, and an open reading frame (ORF) of 318 bp encoding a polypeptide of 105 amino acids. The high similarity of EsTrx1 with Trx1s from other animals indicated that EsTrx1 should be a new member of the Trx1 sub-family. Quantitative real-time PCR analysis revealed the presence of EsTrx1 transcripts in gill, gonad, hepato-pancreas, muscle, heart and haemocytes. The expression of EsTrx1 mRNA in haemocytes was up-regulated after Listonella anguillarum challenge, reached the maximum level at 6 h post-stimulation, and then dropped back to the original level gradually. In order to elucidate its biological functions, EsTrx1 was recombined and expressed in E. coli BL21 (DE3). The rEsTrx1 was demonstrated to possess the expected redox activity in enzymatic analysis, and to be more potent than GSH in antioxidant capacity. These results together indicated that EsTrx1 could function as an important antioxidant in a physiological context, and perhaps is involved in the responses to bacterial challenge. (C) 2009 Elsevier Ltd. All rights reserved.; Thioredoxin, with a redox-active disulfide/dithiol in the active site, is the major ubiquitous disulfide reductase responsible for maintaining proteins in their reduced state. In the present study, the cDNA encoding thioredoxin-1 (designated EsTrx1) was cloned from Chinese mitten crab Eriocheir sinensis by using rapid amplification of cDNA ends (RACE) approaches. The full-length cDNA of EsTrx1 was of 641 bp, containing a 51 untranslated region (UTR) of 17 bp, a 3' UTR of 306 bp with a poly (A) tail, and an open reading frame (ORF) of 318 bp encoding a polypeptide of 105 amino acids. The high similarity of EsTrx1 with Trx1s from other animals indicated that EsTrx1 should be a new member of the Trx1 sub-family. Quantitative real-time PCR analysis revealed the presence of EsTrx1 transcripts in gill, gonad, hepato-pancreas, muscle, heart and haemocytes. The expression of EsTrx1 mRNA in haemocytes was up-regulated after Listonella anguillarum challenge, reached the maximum level at 6 h post-stimulation, and then dropped back to the original level gradually. In order to elucidate its biological functions, EsTrx1 was recombined and expressed in E. coli BL21 (DE3). The rEsTrx1 was demonstrated to possess the expected redox activity in enzymatic analysis, and to be more potent than GSH in antioxidant capacity. These results together indicated that EsTrx1 could function as an important antioxidant in a physiological context, and perhaps is involved in the responses to bacterial challenge. (C) 2009 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000266659400006 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/2991]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.NW A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mu, Changkao,Zhao, Jianmin,Wang, Lingling,et al. A thioredoxin with antioxidant activity identified from Eriocheir sinensis[J]. FISH & SHELLFISH IMMUNOLOGY,2009,26(5):716-723. |
| APA | Mu, Changkao.,Zhao, Jianmin.,Wang, Lingling.,Song, Linsheng.,Song, Xiaoyan.,...&Cui, Zhaoxia.(2009).A thioredoxin with antioxidant activity identified from Eriocheir sinensis.FISH & SHELLFISH IMMUNOLOGY,26(5),716-723. |
| MLA | Mu, Changkao,et al."A thioredoxin with antioxidant activity identified from Eriocheir sinensis".FISH & SHELLFISH IMMUNOLOGY 26.5(2009):716-723. |
入库方式： OAI收割
来源：海洋研究所
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