Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides
文献类型：期刊论文
| 作者 | Niu Jianfeng1; Wang Guangce1,2; Lue Fang1,3; Zhou Baicheng1; Peng Guang1 |
| 刊名 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2009-08-01 |
| 卷号 | 27期号:3页码:502-512 |
| 关键词 | Bryopsis Hypnoides Lectin Hemagglutinating Cell Organelle Aggregation Regeneration |
| ISSN号 | 0254-4059 |
| DOI | 10.1007/s00343-009-9157-4 |
| 文献子类 | Article |
| 英文摘要 | A group of coenocytic marine algae differs from higher plants, whose totipotency depends on an intact cell (or protoplast). Instead, this alga is able to aggregate its extruded protoplasm in sea water and generate new mature individuals. It is thought that lectins play a key role in the aggregation process. We purified a lectin associated with the aggregation of cell organelles in Bryopsis hypnoides. The lectin was ca. 27 kDa with a pI between pH 5 and pH 6. The absence of carbohydrate suggested that the lectin was not a glycoprotein. The hemagglutinating activity (HA) of the lectin was not dependent on the. presence of divalent cations and was inhibited by N-Acetylgalactosamine, N-Acetylglucosamine, and the glycoprotein bovine submaxillary mucin. The lectin preferentially agglutinated Gram-negative bacterium. The HA of this lectin was stable between pH 4 to pH 10. Cell organelles outside the cytoplasm were agglutinated by the addition of lectin solution (0.5 mg ml(-1)). Our results suggest that the regeneration of B. hypnoides is mediated by this lectin. We also demonstrated that the formation of cell organelle aggregates was inhibited by nigericin in natural seawater (pH 8.0). Given that nigericin dissipates proton gradients across the membrane, we hypothesize that the aggregation of cell organelles was proton-gradient dependent.; A group of coenocytic marine algae differs from higher plants, whose totipotency depends on an intact cell (or protoplast). Instead, this alga is able to aggregate its extruded protoplasm in sea water and generate new mature individuals. It is thought that lectins play a key role in the aggregation process. We purified a lectin associated with the aggregation of cell organelles in Bryopsis hypnoides. The lectin was ca. 27 kDa with a pI between pH 5 and pH 6. The absence of carbohydrate suggested that the lectin was not a glycoprotein. The hemagglutinating activity (HA) of the lectin was not dependent on the. presence of divalent cations and was inhibited by N-Acetylgalactosamine, N-Acetylglucosamine, and the glycoprotein bovine submaxillary mucin. The lectin preferentially agglutinated Gram-negative bacterium. The HA of this lectin was stable between pH 4 to pH 10. Cell organelles outside the cytoplasm were agglutinated by the addition of lectin solution (0.5 mg ml(-1)). Our results suggest that the regeneration of B. hypnoides is mediated by this lectin. We also demonstrated that the formation of cell organelle aggregates was inhibited by nigericin in natural seawater (pH 8.0). Given that nigericin dissipates proton gradients across the membrane, we hypothesize that the aggregation of cell organelles was proton-gradient dependent. |
| 学科主题 | Limnology ; Oceanography |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000270742000011 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/2997]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Niu Jianfeng,Wang Guangce,Lue Fang,et al. Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2009,27(3):502-512. |
| APA | Niu Jianfeng,Wang Guangce,Lue Fang,Zhou Baicheng,&Peng Guang.(2009).Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,27(3),502-512. |
| MLA | Niu Jianfeng,et al."Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 27.3(2009):502-512. |
入库方式： OAI收割
来源：海洋研究所
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