A cyclophilin A inducible expressed in gonad of zhikong scallop Chlamys farreri
文献类型：期刊论文
| 作者 | Song, Xiaoyan1,2; Wang, Lingling1; Song, Linsheng1; Zhao, Jianmin1; Zhang, Huan1; Zheng, Peilin1; Qiu, Limei1; Liu, Xiaolin2; Wu, Longtao1 |
| 刊名 | MOLECULAR BIOLOGY REPORTS
 |
| 出版日期 | 2009-07-01 |
| 卷号 | 36期号:6页码:1637-1645 |
| 关键词 | Scallop Chlamys Farreri Cyclophilin a Mrna Expression Quantitative Real-time Pcr Bacteria Challenge |
| ISSN号 | 0301-4851 |
| DOI | 10.1007/s11033-008-9363-8 |
| 文献子类 | Article |
| 英文摘要 | Cyclophilin A (CypA), a receptor for the immunosuppressive agent cyclosporin A (CsA), is a cis-trans peptidyl-prolyl isomerase (PPIase) which accelerates the cis-trans isomerization of prolyl-peptide bonds, interacts with a variety of proteins and therefore regulates their activities. One CypA (designated CfCypA) cDNA was cloned from Chlamys farreri by expressed sequence tag (EST) and rapid amplification of cDNA ends (RACE) techniques. The full-length cDNA of CfCypA consisted of 1,248 nucleotides with a canonical polyadenylation signal sequence AATAAA, a poly (A) tail, and an open reading frame (ORF) of 495 nucleotides encoding a polypeptide of 164 amino acids. The deduced amino acid sequence shared high similarity with CypA from the other species, indicating that CfCypA should be a new member of the CypA family. Quantitative real-time (RT) PCR was employed to assess the mRNA expression of CfCypA in various tissues and its temporal expression in haemocytes and gonad of scallops challenged with Vibrio anguillarum. The mRNA transcripts of CfCypA could be detected in all the examined tissues with highest expression level in gonad. After bacterial challenge, the expression level of CfCypA was almost unchanged in haemocytes, but up-regulated in gonad and increased to the peak (22.59-fold; P < 0.05) at 4 h post-injection, and then dropped to the original level at 8 h post-injection. These results indicated that CfCypA was constitutive expressed in haemocytes, but could be induced in gonad, and perhaps played a critical role in response to the bacterial challenge in gonad.; Cyclophilin A (CypA), a receptor for the immunosuppressive agent cyclosporin A (CsA), is a cis-trans peptidyl-prolyl isomerase (PPIase) which accelerates the cis-trans isomerization of prolyl-peptide bonds, interacts with a variety of proteins and therefore regulates their activities. One CypA (designated CfCypA) cDNA was cloned from Chlamys farreri by expressed sequence tag (EST) and rapid amplification of cDNA ends (RACE) techniques. The full-length cDNA of CfCypA consisted of 1,248 nucleotides with a canonical polyadenylation signal sequence AATAAA, a poly (A) tail, and an open reading frame (ORF) of 495 nucleotides encoding a polypeptide of 164 amino acids. The deduced amino acid sequence shared high similarity with CypA from the other species, indicating that CfCypA should be a new member of the CypA family. Quantitative real-time (RT) PCR was employed to assess the mRNA expression of CfCypA in various tissues and its temporal expression in haemocytes and gonad of scallops challenged with Vibrio anguillarum. The mRNA transcripts of CfCypA could be detected in all the examined tissues with highest expression level in gonad. After bacterial challenge, the expression level of CfCypA was almost unchanged in haemocytes, but up-regulated in gonad and increased to the peak (22.59-fold; P < 0.05) at 4 h post-injection, and then dropped to the original level at 8 h post-injection. These results indicated that CfCypA was constitutive expressed in haemocytes, but could be induced in gonad, and perhaps played a critical role in response to the bacterial challenge in gonad. |
| 学科主题 | Biochemistry & Molecular Biology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000266454500061 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3011]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China 2.NW A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Xiaoyan,Wang, Lingling,Song, Linsheng,et al. A cyclophilin A inducible expressed in gonad of zhikong scallop Chlamys farreri[J]. MOLECULAR BIOLOGY REPORTS,2009,36(6):1637-1645. |
| APA | Song, Xiaoyan.,Wang, Lingling.,Song, Linsheng.,Zhao, Jianmin.,Zhang, Huan.,...&Wu, Longtao.(2009).A cyclophilin A inducible expressed in gonad of zhikong scallop Chlamys farreri.MOLECULAR BIOLOGY REPORTS,36(6),1637-1645. |
| MLA | Song, Xiaoyan,et al."A cyclophilin A inducible expressed in gonad of zhikong scallop Chlamys farreri".MOLECULAR BIOLOGY REPORTS 36.6(2009):1637-1645. |
入库方式： OAI收割
来源：海洋研究所
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