Domain analysis of the Edwardsiella tarda ferric uptake regulator
文献类型：期刊论文
| 作者 | Sun, Kun1,2; Cheng, Shuang1,2; Wang, Fang1,2,3; Sun, Li1 |
| 刊名 | JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
 |
| 出版日期 | 2009-10-01 |
| 卷号 | 55期号:5页码:351-358 |
| 关键词 | Domain Swapping Edwardsiella Tarda Ferric Uptake Regulator Mutagenesis |
| ISSN号 | 0022-1260 |
| 文献子类 | Article |
| 英文摘要 | Recent studies have shown that the ferric uptake regulator (Fur) of Edwardsiella tarda (Fur(Et)) shares high sequence identity with the Escherichia coli Fur (Fur(Ec)) at the N-terminal DNA-binding region. In the present study, the functional importance of the C-terminal region of Fur(Et) was investigated. It was found that Fur(Et) bearing deletion of the C-terminal 12 residues still possesses most of the repressor activity, whereas Fur(Et) bearing deletions of the C-terminal 16 and more than 16 residues are severely affected in activity. Domain swapping analyses indicated that the chimeric Fur proteins (Et75Ec73 and Et75Vh74) consisting of the N-terminal 1-75 region of Fur(Et) fused to the C-terminal 76-148 region of Fur(Ec) and the C-terminal 76-149 region of the Vibrio harveyi Fur (Fur(Vh)), respectively, are fully active. C92 of Fur(Ec) and C137 of Fur(Vh), which are functionally essential in Fur(Ec) and Fur(Vh), respectively, are also essential in Et75Ec73 and Et75074, respectively. Further study identified an artificial Fur protein, EtMF54, which is composed of the N-terminal 49 residues of Fur(Et) and five artificial residues. Compared to Fur(Et), EtMF54 possesses partial Fur activity that is iron-dependent. These results (I) indicate that there exist certain functional/structural compatibilities among Fur(Et), Fur(Ec), and Fur(Vh) at the C-terminal region; (ii) provide insights to the potential location of the regulatory ion-binding site of Fur(Et).; Recent studies have shown that the ferric uptake regulator (Fur) of Edwardsiella tarda (Fur(Et)) shares high sequence identity with the Escherichia coli Fur (Fur(Ec)) at the N-terminal DNA-binding region. In the present study, the functional importance of the C-terminal region of Fur(Et) was investigated. It was found that Fur(Et) bearing deletion of the C-terminal 12 residues still possesses most of the repressor activity, whereas Fur(Et) bearing deletions of the C-terminal 16 and more than 16 residues are severely affected in activity. Domain swapping analyses indicated that the chimeric Fur proteins (Et75Ec73 and Et75Vh74) consisting of the N-terminal 1-75 region of Fur(Et) fused to the C-terminal 76-148 region of Fur(Ec) and the C-terminal 76-149 region of the Vibrio harveyi Fur (Fur(Vh)), respectively, are fully active. C92 of Fur(Ec) and C137 of Fur(Vh), which are functionally essential in Fur(Ec) and Fur(Vh), respectively, are also essential in Et75Ec73 and Et75074, respectively. Further study identified an artificial Fur protein, EtMF54, which is composed of the N-terminal 49 residues of Fur(Et) and five artificial residues. Compared to Fur(Et), EtMF54 possesses partial Fur activity that is iron-dependent. These results (I) indicate that there exist certain functional/structural compatibilities among Fur(Et), Fur(Ec), and Fur(Vh) at the C-terminal region; (ii) provide insights to the potential location of the regulatory ion-binding site of Fur(Et). |
| 学科主题 | Biotechnology & Applied Microbiology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000272759000006 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3013]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Kun,Cheng, Shuang,Wang, Fang,et al. Domain analysis of the Edwardsiella tarda ferric uptake regulator[J]. JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY,2009,55(5):351-358. |
| APA | Sun, Kun,Cheng, Shuang,Wang, Fang,&Sun, Li.(2009).Domain analysis of the Edwardsiella tarda ferric uptake regulator.JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY,55(5),351-358. |
| MLA | Sun, Kun,et al."Domain analysis of the Edwardsiella tarda ferric uptake regulator".JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY 55.5(2009):351-358. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。