Immunoprotective analysis of VhhP2, a Vibrio harveyi vaccine candidate
文献类型：期刊论文
| 作者 | Sun, Kun1,2; Zhang, Wei-wei1,2; Hou, Jin-hui3; Sun, Li1 |
| 刊名 | VACCINE
 |
| 出版日期 | 2009-05-11 |
| 卷号 | 27期号:21页码:2733-2740 |
| 关键词 | Cross-protection Immune Response Surface Display Vaccine Vibrio Harveyi |
| ISSN号 | 0264-410X |
| DOI | 10.1016/j.vaccine.2009.03.012 |
| 文献子类 | Article |
| 英文摘要 | VhhP2 is an Outer membrane protein identified in a pathogenic Vibrio harveyi strain, T4, isolated from diseased fish. When used as a Subunit Vaccine, purified recombinant VhhP2 affords high level of protection upon Japanese flounder against V harveyi challenge. Vaccination with VhhP2 induced the expression of a number of immune-related genes, especially those encoding immunoglobulin M (IgM) and major histocompatibility complex (MHC) II alpha. A VhhP2 surface display system, in the form of the fish commensal strain FIR harboring the vhhP2-expressing plasmid pJVP, was constructed. PF3/pJVP is able to produce and present recombinant VhhP2 on cell surface. Vaccination of fish with live PF3/pJVP via intraperitoneal injection elicited Strong immunoprotection. Vaccination of fish orally with live PF3/pJVP embedded in alginate microspheres also induced effective immunoprotection. In addition, a VhhP2-based surface display system was created, in which VhhP2 serves as a carrier for the Surface delivery of a heterologous Edwardsiella tarda immunogen, Et18, that is fused in-frame to VhhP2. DH5 alpha/pJVP18, which expresses and surface-displays the VhhP2-Et18 chimera, proved to be an effective vaccine that call protect fish against infections by V. harveyi and E. tarda to the extents comparable to those produced by vaccination with purified recombinant VhhP2 and Et18, respectively. These data suggest that VhhP2 may be applied as a vaccine and a vaccine carrier against infections by V. harveyi and other pathogens such as F. tarda. (C) 2009 Elsevier Ltd. All rights reserved.; VhhP2 is an Outer membrane protein identified in a pathogenic Vibrio harveyi strain, T4, isolated from diseased fish. When used as a Subunit Vaccine, purified recombinant VhhP2 affords high level of protection upon Japanese flounder against V harveyi challenge. Vaccination with VhhP2 induced the expression of a number of immune-related genes, especially those encoding immunoglobulin M (IgM) and major histocompatibility complex (MHC) II alpha. A VhhP2 surface display system, in the form of the fish commensal strain FIR harboring the vhhP2-expressing plasmid pJVP, was constructed. PF3/pJVP is able to produce and present recombinant VhhP2 on cell surface. Vaccination of fish with live PF3/pJVP via intraperitoneal injection elicited Strong immunoprotection. Vaccination of fish orally with live PF3/pJVP embedded in alginate microspheres also induced effective immunoprotection. In addition, a VhhP2-based surface display system was created, in which VhhP2 serves as a carrier for the Surface delivery of a heterologous Edwardsiella tarda immunogen, Et18, that is fused in-frame to VhhP2. DH5 alpha/pJVP18, which expresses and surface-displays the VhhP2-Et18 chimera, proved to be an effective vaccine that call protect fish against infections by V. harveyi and E. tarda to the extents comparable to those produced by vaccination with purified recombinant VhhP2 and Et18, respectively. These data suggest that VhhP2 may be applied as a vaccine and a vaccine carrier against infections by V. harveyi and other pathogens such as F. tarda. (C) 2009 Elsevier Ltd. All rights reserved. |
| 学科主题 | Immunology ; Medicine, Research & Experimental |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000266023100002 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3019]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.Xuzhou Inst Technol, Xuzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Kun,Zhang, Wei-wei,Hou, Jin-hui,et al. Immunoprotective analysis of VhhP2, a Vibrio harveyi vaccine candidate[J]. VACCINE,2009,27(21):2733-2740. |
| APA | Sun, Kun,Zhang, Wei-wei,Hou, Jin-hui,&Sun, Li.(2009).Immunoprotective analysis of VhhP2, a Vibrio harveyi vaccine candidate.VACCINE,27(21),2733-2740. |
| MLA | Sun, Kun,et al."Immunoprotective analysis of VhhP2, a Vibrio harveyi vaccine candidate".VACCINE 27.21(2009):2733-2740. |
入库方式： OAI收割
来源：海洋研究所
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