Purification and characterization of the chitosanase from Aeromonas sp HG08
文献类型：期刊论文
| 作者 | Sun, Yuying2; Zhang, Jiquan1; Wang, Shujun2 |
| 刊名 | AFRICAN JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2009-06-17 |
| 卷号 | 8期号:12页码:2830-2834 |
| 关键词 | Aeromonas Sp. Chitosanase Purification Characterization |
| ISSN号 | 1684-5315 |
| 文献子类 | Article |
| 英文摘要 | In this work, the characterization of a chitosanase-producing bacterium isolated from soil was reported and this strain was grouped under the genus Aeromonas by virtue of its morphological, physiological properties and 16S rDNA gene sequences. It is the first report that the genus Aeromonas could produce chitosanase. Aeromonas sp. HG08 could secrete the chitosanase ( named AsChi) with molecular weight of 70 kDa. The optimum pH and temperature of AsChi was 6.0 and 55 degrees C, respectively. The activity of AsChi was markedly enhanced by Mn(2+) and inhibited by Fe(3+), Cu(2+), Ag(+) and Hg(2+); additionally, the activity of AsChi was increased with the degree of deacetylation ( DDA) of chitosan. Through viscosimetric assay, AsChi probably hydrolyzed chitosan in an endo-type fashion.; In this work, the characterization of a chitosanase-producing bacterium isolated from soil was reported and this strain was grouped under the genus Aeromonas by virtue of its morphological, physiological properties and 16S rDNA gene sequences. It is the first report that the genus Aeromonas could produce chitosanase. Aeromonas sp. HG08 could secrete the chitosanase ( named AsChi) with molecular weight of 70 kDa. The optimum pH and temperature of AsChi was 6.0 and 55 degrees C, respectively. The activity of AsChi was markedly enhanced by Mn2+ and inhibited by Fe3+, Cu2+, Ag+ and Hg2+; additionally, the activity of AsChi was increased with the degree of deacetylation ( DDA) of chitosan. Through viscosimetric assay, AsChi probably hydrolyzed chitosan in an endo-type fashion. |
| 学科主题 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000268831200028 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3021]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Huaihai Inst Technol, Coll Marine Sci, Lianyungang 222005, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Yuying,Zhang, Jiquan,Wang, Shujun. Purification and characterization of the chitosanase from Aeromonas sp HG08[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2009,8(12):2830-2834. |
| APA | Sun, Yuying,Zhang, Jiquan,&Wang, Shujun.(2009).Purification and characterization of the chitosanase from Aeromonas sp HG08.AFRICAN JOURNAL OF BIOTECHNOLOGY,8(12),2830-2834. |
| MLA | Sun, Yuying,et al."Purification and characterization of the chitosanase from Aeromonas sp HG08".AFRICAN JOURNAL OF BIOTECHNOLOGY 8.12(2009):2830-2834. |
入库方式： OAI收割
来源：海洋研究所
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