Expressed sequence tags from the zhikong scallop (Chlamys farreri): Discovery and annotation of host-defense genes
文献类型：期刊论文
| 作者 | Wang, Lingling1; Song, Linsheng1; Zhao, Jianmin1; Qiu, Limei1; Zhang, Huan1; Xu, Wei1; Li, Honglei1 ; Li, Chenhua1; Wu, Longtao1; Guo, Ximing2
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| 刊名 | FISH & SHELLFISH IMMUNOLOGY
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| 出版日期 | 2009-05-01 |
| 卷号 | 26期号:5页码:744-750 |
| 关键词 | Chlamys Farreri Cdna Library Expressed Sequence Tags Bacteria Challenge Functional Annotation Host-defense Genes |
| ISSN号 | 1050-4648 |
| DOI | 10.1016/j.fsi.2009.03.002 |
| 文献子类 | Article |
| 英文摘要 | A high-quality cDNA library was constructed from whole body tissues of the zhikong scallop, Chlamys farreri, challenged by Listonella anguillarum. A total of 5720 clones were sequenced, yielding 5123 expressed sequence tags (ESTs). Among the 3326 unique genes identified, 2289 (69%) genes had no significant (E-value < 1e-5) matches to known sequences in public databases and 194 (6%) matched proteins of unknown functions. The remaining 843 (25%) genes that exhibited homology with genes of known functions, showed broad involvement in metabolic processes (31%), cell structure and motility (20%), gene and protein expression (12%), cell signaling and cell communication (8%), cell division (4%), and notably, 25% of those genes were related to immune function. They included stress response genes, complement-like genes, proteinase and proteinase inhibitors, immune recognition receptors and immune effectors. The EST collection obtained in this study provides a useful resource for gene discovery and especially for the identification of host-defense genes and systems in scallops and other molluscs. (C) 2009 Elsevier Ltd. All rights reserved.; A high-quality cDNA library was constructed from whole body tissues of the zhikong scallop, Chlamys farreri, challenged by Listonella anguillarum. A total of 5720 clones were sequenced, yielding 5123 expressed sequence tags (ESTs). Among the 3326 unique genes identified, 2289 (69%) genes had no significant (E-value < 1e-5) matches to known sequences in public databases and 194 (6%) matched proteins of unknown functions. The remaining 843 (25%) genes that exhibited homology with genes of known functions, showed broad involvement in metabolic processes (31%), cell structure and motility (20%), gene and protein expression (12%), cell signaling and cell communication (8%), cell division (4%), and notably, 25% of those genes were related to immune function. They included stress response genes, complement-like genes, proteinase and proteinase inhibitors, immune recognition receptors and immune effectors. The EST collection obtained in this study provides a useful resource for gene discovery and especially for the identification of host-defense genes and systems in scallops and other molluscs. (C) 2009 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000266659400010 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3033]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA |
| 推荐引用方式 GB/T 7714 | Wang, Lingling,Song, Linsheng,Zhao, Jianmin,et al. Expressed sequence tags from the zhikong scallop (Chlamys farreri): Discovery and annotation of host-defense genes[J]. FISH & SHELLFISH IMMUNOLOGY,2009,26(5):744-750. |
| APA | Wang, Lingling.,Song, Linsheng.,Zhao, Jianmin.,Qiu, Limei.,Zhang, Huan.,...&Guo, Ximing.(2009).Expressed sequence tags from the zhikong scallop (Chlamys farreri): Discovery and annotation of host-defense genes.FISH & SHELLFISH IMMUNOLOGY,26(5),744-750. |
| MLA | Wang, Lingling,et al."Expressed sequence tags from the zhikong scallop (Chlamys farreri): Discovery and annotation of host-defense genes".FISH & SHELLFISH IMMUNOLOGY 26.5(2009):744-750. |
入库方式： OAI收割
来源：海洋研究所
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