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Chinese Academy of Sciences Institutional Repositories Grid
VEGF gene silencing by cytomegalovirus promoter driven ShRNA expression vector results in vascular development defects in zebrafish

文献类型:期刊论文

作者Yang, S. L.1; Yan, S.3; Niu, R. L.2; Lin, X. K.2
刊名RUSSIAN JOURNAL OF GENETICS
出版日期2009-09-01
卷号45期号:9页码:1040-1046
ISSN号1022-7954
关键词Endothelial Growth-factor Pol-ii Promoter Rna Interference Angiogenesis Hematopoiesis Neuropilin-1 Mammals System Cell
DOI10.1134/S1022795409090038
文献子类Article
英文摘要Zebrafish has been generally considered as an excellent model in case of drug screening, disease model establishment, and vertebrate embryonic development study. In this work, the ability of human cytomegalovirus immediate early promoter (CMV promoter)-driven short hairpin RNA (shRNA) expression vector to induce shRNA against VEGF gene in zebrafish was tested, and its effect on vascular development was assed, too. Using RT-qPCR, blood vessel staining, and in situ hybridization, we confirmed certain transcriptional activity and down regulation of gene expression by the vector. In situ hybridization analysis indicated selective inhibition of NRP1 expression in the VEGF gene loss of function model, which might imply in turn that VEGF could not only activate endothelial cells directly but also could contribute to stimulating angiogenesis in vivo by a mechanism that involved up-regulation of its cognate receptor expression in zebrafish. This contributed to a better understanding of molecular mechanisms of cardiovascular development. The system improved the success rate in making inducible knockdown and widened the possibilities for better therapeutic targets in zebrafish.; Zebrafish has been generally considered as an excellent model in case of drug screening, disease model establishment, and vertebrate embryonic development study. In this work, the ability of human cytomegalovirus immediate early promoter (CMV promoter)-driven short hairpin RNA (shRNA) expression vector to induce shRNA against VEGF gene in zebrafish was tested, and its effect on vascular development was assed, too. Using RT-qPCR, blood vessel staining, and in situ hybridization, we confirmed certain transcriptional activity and down regulation of gene expression by the vector. In situ hybridization analysis indicated selective inhibition of NRP1 expression in the VEGF gene loss of function model, which might imply in turn that VEGF could not only activate endothelial cells directly but also could contribute to stimulating angiogenesis in vivo by a mechanism that involved up-regulation of its cognate receptor expression in zebrafish. This contributed to a better understanding of molecular mechanisms of cardiovascular development. The system improved the success rate in making inducible knockdown and widened the possibilities for better therapeutic targets in zebrafish.
学科主题Genetics & Heredity
URL标识查看原文
语种英语
WOS记录号WOS:000269974100003
公开日期2010-12-22
源URL[http://ir.qdio.ac.cn/handle/337002/3053]  
专题海洋研究所_实验海洋生物学重点实验室
作者单位1.CAS, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China
2.CAS, Inst Oceanol, Key Lab Marine Biol, Qingdao 266071, Peoples R China
3.Dalian Jiaotong Univ, Coll Environm & Chem Engn, Dalian 116028, Peoples R China
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GB/T 7714
Yang, S. L.,Yan, S.,Niu, R. L.,et al. VEGF gene silencing by cytomegalovirus promoter driven ShRNA expression vector results in vascular development defects in zebrafish[J]. RUSSIAN JOURNAL OF GENETICS,2009,45(9):1040-1046.
APA Yang, S. L.,Yan, S.,Niu, R. L.,&Lin, X. K..(2009).VEGF gene silencing by cytomegalovirus promoter driven ShRNA expression vector results in vascular development defects in zebrafish.RUSSIAN JOURNAL OF GENETICS,45(9),1040-1046.
MLA Yang, S. L.,et al."VEGF gene silencing by cytomegalovirus promoter driven ShRNA expression vector results in vascular development defects in zebrafish".RUSSIAN JOURNAL OF GENETICS 45.9(2009):1040-1046.

入库方式: OAI收割

来源:海洋研究所

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