Improved RNA isolation from Laminaria japonica Aresch (Laminariaceae, Phaeophyta)
文献类型：期刊论文
| 作者 | Yao, Jianting1,2; Fu, Wandong1,2; Wang, Xiuliang1; Duan, Delin1 |
| 刊名 | JOURNAL OF APPLIED PHYCOLOGY
 |
| 出版日期 | 2009-04-01 |
| 卷号 | 21期号:2页码:233-238 |
| 关键词 | Phaeophyta Phenolics Ctab Polysaccharides Rna Extraction |
| ISSN号 | 0921-8971 |
| DOI | 10.1007/s10811-008-9354-0 |
| 文献子类 | Article |
| 英文摘要 | RNA isolation is difficult in some plants and algae because phenolics, polysaccharides, or other compounds can bind or co-precipitate with RNA, and because the success of RNA isolation can be strain-specific and species-specific. To create an improved RNA isolation protocol for Laminaria japonica Aresch (Laminariaceae, Phaeophyta), four methods for extracting RNA were tested. A cetyltrimethylammonium bromide (CTAB)-based RNA extraction protocol was developed that clearly showed 28S and 18S ribosomal RNA bands and produced RNA with high yield (68 mu g g(-1) fresh weight) and high quality (A (260/280) ratio 1.96 +/- 0.05). The isolated RNA was intact, and RT-PCR analysis confirmed that further molecular application is feasible.; RNA isolation is difficult in some plants and algae because phenolics, polysaccharides, or other compounds can bind or co-precipitate with RNA, and because the success of RNA isolation can be strain-specific and species-specific. To create an improved RNA isolation protocol for Laminaria japonica Aresch (Laminariaceae, Phaeophyta), four methods for extracting RNA were tested. A cetyltrimethylammonium bromide (CTAB)-based RNA extraction protocol was developed that clearly showed 28S and 18S ribosomal RNA bands and produced RNA with high yield (68 mu g g(-1) fresh weight) and high quality (A (260/280) ratio 1.96 +/- 0.05). The isolated RNA was intact, and RT-PCR analysis confirmed that further molecular application is feasible. |
| 学科主题 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000264483600009 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3061]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100059, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yao, Jianting,Fu, Wandong,Wang, Xiuliang,et al. Improved RNA isolation from Laminaria japonica Aresch (Laminariaceae, Phaeophyta)[J]. JOURNAL OF APPLIED PHYCOLOGY,2009,21(2):233-238. |
| APA | Yao, Jianting,Fu, Wandong,Wang, Xiuliang,&Duan, Delin.(2009).Improved RNA isolation from Laminaria japonica Aresch (Laminariaceae, Phaeophyta).JOURNAL OF APPLIED PHYCOLOGY,21(2),233-238. |
| MLA | Yao, Jianting,et al."Improved RNA isolation from Laminaria japonica Aresch (Laminariaceae, Phaeophyta)".JOURNAL OF APPLIED PHYCOLOGY 21.2(2009):233-238. |
入库方式： OAI收割
来源：海洋研究所
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