Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses
文献类型：期刊论文
| 作者 | You Feng1; Wang Wei1; Xu Dongdong1; Zhu Xiangping1,2; Ni Jing1; Wu Zhihao1; Xu Yongli1; Wang Xincheng1; Zhang Peijun1 |
| 刊名 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2009-06-01 |
| 卷号 | 27期号:2页码:317-323 |
| 关键词 | Artificial Hybridization Paralichthys Olivaceus (Temminck Et Schlegel) Kareius Bicoloratus (Basilewsky) Karyotype Allozyme Rapd |
| ISSN号 | 0254-4059 |
| DOI | 10.1007/s00343-009-9134-y |
| 文献子类 | Article |
| 英文摘要 | The hybrid between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus was produced by artificial insemination of olive flounder eggs with stone flounder sperm. Sinistral and dextral are two types of hybrid progeny after metamorphosis. Karyotypes of both hybrid flounders are the same as those of the two parental species. Of the 22 loci examined from 12 allozymes, 12 confirmed hybridization of the paternal and maternal loci in hybrids and no difference was found in allozyme patterns of sinistral and dextral hybrid fishes. RAPD patterns of these specimens were also studied with 38 primers selected from 104 tested. Among them, the PCR products of 30 primers showed hybridization of the paternal and maternal bands. Genetic variation between hybrids and their parental stocks was analyzed by RAPD using 10 of the above 38 primers. The average heterozygosity and genetic distance were calculated. The results suggested that the filial generation could inherit a little more genetic materials from paternal fish than that from maternal fish.; The hybrid between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus was produced by artificial insemination of olive flounder eggs with stone flounder sperm. Sinistral and dextral are two types of hybrid progeny after metamorphosis. Karyotypes of both hybrid flounders are the same as those of the two parental species. Of the 22 loci examined from 12 allozymes, 12 confirmed hybridization of the paternal and maternal loci in hybrids and no difference was found in allozyme patterns of sinistral and dextral hybrid fishes. RAPD patterns of these specimens were also studied with 38 primers selected from 104 tested. Among them, the PCR products of 30 primers showed hybridization of the paternal and maternal bands. Genetic variation between hybrids and their parental stocks was analyzed by RAPD using 10 of the above 38 primers. The average heterozygosity and genetic distance were calculated. The results suggested that the filial generation could inherit a little more genetic materials from paternal fish than that from maternal fish. |
| 学科主题 | Limnology ; Oceanography |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000267970300016 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3063]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Qingdao Agr Univ, Qingdao 266109, Peoples R China |
| 推荐引用方式 GB/T 7714 | You Feng,Wang Wei,Xu Dongdong,et al. Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2009,27(2):317-323. |
| APA | You Feng.,Wang Wei.,Xu Dongdong.,Zhu Xiangping.,Ni Jing.,...&Zhang Peijun.(2009).Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,27(2),317-323. |
| MLA | You Feng,et al."Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 27.2(2009):317-323. |
入库方式： OAI收割
来源：海洋研究所
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