A novel C-type lectin (Cflec-3) from Chlamys farreri with three carbohydrate-recognition domains
文献类型：期刊论文
| 作者 | Zhang, Huan1,2; Wang, Hao1; Wang, Lingling1; Song, Xiaoyan3; Zhao, Jianmin1; Qiu, Limei1; Li, Ling1,2; Cong, Ming1; Song, Linsheng1 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2009-05-01 |
| 卷号 | 26期号:5页码:707-715 |
| 关键词 | Chlamys Farreri C-type Lectin Innate Immunity Bacterial Agglutination Recombinant Expression Real-time Rt-pcr |
| ISSN号 | 1050-4648 |
| DOI | 10.1016/j.fsi.2009.02.017 |
| 文献子类 | Article |
| 英文摘要 | C-type lectins are a superfamily of carbohydrate-recognition proteins which play crucial roles in the innate immunity. In this study, the gene of a C-type lectin with multiple carbohydrate-recognition domains (CRDs) from scallop Chlamys farreri (designated as Cflec-3) was cloned by rapid amplification of cDNA ends (RACE) approach based on expression sequence tag (EST) analysis. The full-length cDNA of Cflec-3 was of 2256 bp. The open reading frame encoded a polypeptide of 516 amino acids, including a signal sequence and three CRDs. The deduced amino acid sequence of Cflec-3 showed high similarity to members of C-type lectin superfamily. By fluorescent quantitative real-time PCR, the Cflec-3 mRNA was mainly detected in hepatopancreas, adductor, mantle, and marginally in gill, gonad and hemocytes of healthy scallops. After scallops were challenged by Listonella anguillarum, the mRNA level of Cflec-3 in hemocytes was up-regulated and was significantly higher than that of blank at 8 h and 12 h post-challenge. The function of Cflec-3 was investigated by recombination and expression of the cDNA fragment encoding its mature peptide in Escherichia coli BL21 (DE3)-pLysS. The recombined Cflec-3 (rCflec-3) agglutinated Gram-negative bacteria Pseudomonas stutzeri. The agglutinating activity was calcium-dependent and could be inhibited by D-mannose. These results collectively suggested that Cflec-3 was involved in the immune response against microbe infection and contributed to nonself-recognition and clearance of bacterial pathogens in scallop. (C) 2009 Elsevier Ltd. All rights reserved.; C-type lectins are a superfamily of carbohydrate-recognition proteins which play crucial roles in the innate immunity. In this study, the gene of a C-type lectin with multiple carbohydrate-recognition domains (CRDs) from scallop Chlamys farreri (designated as Cflec-3) was cloned by rapid amplification of cDNA ends (RACE) approach based on expression sequence tag (EST) analysis. The full-length cDNA of Cflec-3 was of 2256 bp. The open reading frame encoded a polypeptide of 516 amino acids, including a signal sequence and three CRDs. The deduced amino acid sequence of Cflec-3 showed high similarity to members of C-type lectin superfamily. By fluorescent quantitative real-time PCR, the Cflec-3 mRNA was mainly detected in hepatopancreas, adductor, mantle, and marginally in gill, gonad and hemocytes of healthy scallops. After scallops were challenged by Listonella anguillarum, the mRNA level of Cflec-3 in hemocytes was up-regulated and was significantly higher than that of blank at 8 h and 12 h post-challenge. The function of Cflec-3 was investigated by recombination and expression of the cDNA fragment encoding its mature peptide in Escherichia coli BL21 (DE3)-pLysS. The recombined Cflec-3 (rCflec-3) agglutinated Gram-negative bacteria Pseudomonas stutzeri. The agglutinating activity was calcium-dependent and could be inhibited by D-mannose. These results collectively suggested that Cflec-3 was involved in the immune response against microbe infection and contributed to nonself-recognition and clearance of bacterial pathogens in scallop. (C) 2009 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000266659400005 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3069]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.NW A&F Univ, Yangling 712100, Shaanxi, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Huan,Wang, Hao,Wang, Lingling,et al. A novel C-type lectin (Cflec-3) from Chlamys farreri with three carbohydrate-recognition domains[J]. FISH & SHELLFISH IMMUNOLOGY,2009,26(5):707-715. |
| APA | Zhang, Huan.,Wang, Hao.,Wang, Lingling.,Song, Xiaoyan.,Zhao, Jianmin.,...&Song, Linsheng.(2009).A novel C-type lectin (Cflec-3) from Chlamys farreri with three carbohydrate-recognition domains.FISH & SHELLFISH IMMUNOLOGY,26(5),707-715. |
| MLA | Zhang, Huan,et al."A novel C-type lectin (Cflec-3) from Chlamys farreri with three carbohydrate-recognition domains".FISH & SHELLFISH IMMUNOLOGY 26.5(2009):707-715. |
入库方式： OAI收割
来源：海洋研究所
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