中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Biosynthesis of fluorescent allophycocyanin alpha-subunits by autocatalysis in Escherichia coli

文献类型:期刊论文

作者Zhang, Weijie1,2; Guan, Xiangyu3; Yang, Yu1,2; Ge, Baosheng4; Chen, Huaxin1; Li, Fuchao1; Qin, Song1
刊名BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
出版日期2009-02-01
卷号52页码:135-140
关键词Autocatalysis Escherichia Coli Fluorescence Recombinant Apo-allophycocyanin Alpha-subunit Recombinant Holo-allophycocyanin Alpha-subunit
ISSN号0885-4513
DOI10.1042/BA20070187
文献子类Article
英文摘要APC (allophycocyanin) is widely used for fluorescence tagging and may be a promising antioxidant agent for use within the food and pharmaceutical industries. Chromophore attachment to apo-ApcA (apo-APC alpha-subunit without chromophore) can be auto-catalysed both in vitro and in vivo. In the present study, a plasmid containing genes of apo-ApcA and chromophore synthetases (HOI (ferredoxin-dependent haem oxygenase) and PcyA (phycocyanobilin:ferredoxin oxidoreductase)] was constructed and expressed in Escherichia coli. The results show that holo-ApcA (APC alpha-subunit with chromophore) can be synthesized by autocatalysis in E. coli. Recombinant holo-ApcA showed the same spectral and fluorescent properties as PC (phycocyanin) and could serve as a good substitute for native PC for fluorescent tagging. Moreover, recombinant ApcA can inhibit hydroxyl and peroxyl radicals more strongly than holo-ApcA and native APC. The EC50 values were 296.4 +/- 22.4 mu g/ml against hydroxyl radicals and 38.5 +/- 2.6 mu g/ml against peroxyl radicals.; APC (allophycocyanin) is widely used for fluorescence tagging and may be a promising antioxidant agent for use within the food and pharmaceutical industries. Chromophore attachment to apo-ApcA (apo-APC alpha-subunit without chromophore) can be auto-catalysed both in vitro and in vivo. In the present study, a plasmid containing genes of apo-ApcA and chromophore synthetases (HOI (ferredoxin-dependent haem oxygenase) and PcyA (phycocyanobilin:ferredoxin oxidoreductase)] was constructed and expressed in Escherichia coli. The results show that holo-ApcA (APC alpha-subunit with chromophore) can be synthesized by autocatalysis in E. coli. Recombinant holo-ApcA showed the same spectral and fluorescent properties as PC (phycocyanin) and could serve as a good substitute for native PC for fluorescent tagging. Moreover, recombinant ApcA can inhibit hydroxyl and peroxyl radicals more strongly than holo-ApcA and native APC. The EC50 values were 296.4 +/- 22.4 mu g/ml against hydroxyl radicals and 38.5 +/- 2.6 mu g/ml against peroxyl radicals.
学科主题Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
URL标识查看原文
语种英语
WOS记录号WOS:000263202500005
公开日期2010-12-22
源URL[http://ir.qdio.ac.cn/handle/337002/3071]  
专题海洋研究所_实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Key Lab Expt Marin Biol, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
3.Ocean Univ China, Fac Life Sci & Technol, Coll Marine Life Sci, Qingdao 266003, Peoples R China
4.China Univ Petr, Ctr Biotechnol & Bioengn, Qingdao 266555, Shandong, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Weijie,Guan, Xiangyu,Yang, Yu,et al. Biosynthesis of fluorescent allophycocyanin alpha-subunits by autocatalysis in Escherichia coli[J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,2009,52:135-140.
APA Zhang, Weijie.,Guan, Xiangyu.,Yang, Yu.,Ge, Baosheng.,Chen, Huaxin.,...&Qin, Song.(2009).Biosynthesis of fluorescent allophycocyanin alpha-subunits by autocatalysis in Escherichia coli.BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,52,135-140.
MLA Zhang, Weijie,et al."Biosynthesis of fluorescent allophycocyanin alpha-subunits by autocatalysis in Escherichia coli".BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 52(2009):135-140.

入库方式: OAI收割

来源:海洋研究所

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