Biosynthesis of fluorescent allophycocyanin alpha-subunits by autocatalysis in Escherichia coli
文献类型:期刊论文
作者 | Zhang, Weijie1,2; Guan, Xiangyu3; Yang, Yu1,2; Ge, Baosheng4; Chen, Huaxin1; Li, Fuchao1; Qin, Song1 |
刊名 | BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
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出版日期 | 2009-02-01 |
卷号 | 52页码:135-140 |
关键词 | Autocatalysis Escherichia Coli Fluorescence Recombinant Apo-allophycocyanin Alpha-subunit Recombinant Holo-allophycocyanin Alpha-subunit |
ISSN号 | 0885-4513 |
DOI | 10.1042/BA20070187 |
文献子类 | Article |
英文摘要 | APC (allophycocyanin) is widely used for fluorescence tagging and may be a promising antioxidant agent for use within the food and pharmaceutical industries. Chromophore attachment to apo-ApcA (apo-APC alpha-subunit without chromophore) can be auto-catalysed both in vitro and in vivo. In the present study, a plasmid containing genes of apo-ApcA and chromophore synthetases (HOI (ferredoxin-dependent haem oxygenase) and PcyA (phycocyanobilin:ferredoxin oxidoreductase)] was constructed and expressed in Escherichia coli. The results show that holo-ApcA (APC alpha-subunit with chromophore) can be synthesized by autocatalysis in E. coli. Recombinant holo-ApcA showed the same spectral and fluorescent properties as PC (phycocyanin) and could serve as a good substitute for native PC for fluorescent tagging. Moreover, recombinant ApcA can inhibit hydroxyl and peroxyl radicals more strongly than holo-ApcA and native APC. The EC50 values were 296.4 +/- 22.4 mu g/ml against hydroxyl radicals and 38.5 +/- 2.6 mu g/ml against peroxyl radicals.; APC (allophycocyanin) is widely used for fluorescence tagging and may be a promising antioxidant agent for use within the food and pharmaceutical industries. Chromophore attachment to apo-ApcA (apo-APC alpha-subunit without chromophore) can be auto-catalysed both in vitro and in vivo. In the present study, a plasmid containing genes of apo-ApcA and chromophore synthetases (HOI (ferredoxin-dependent haem oxygenase) and PcyA (phycocyanobilin:ferredoxin oxidoreductase)] was constructed and expressed in Escherichia coli. The results show that holo-ApcA (APC alpha-subunit with chromophore) can be synthesized by autocatalysis in E. coli. Recombinant holo-ApcA showed the same spectral and fluorescent properties as PC (phycocyanin) and could serve as a good substitute for native PC for fluorescent tagging. Moreover, recombinant ApcA can inhibit hydroxyl and peroxyl radicals more strongly than holo-ApcA and native APC. The EC50 values were 296.4 +/- 22.4 mu g/ml against hydroxyl radicals and 38.5 +/- 2.6 mu g/ml against peroxyl radicals. |
学科主题 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
URL标识 | 查看原文 |
语种 | 英语 |
WOS记录号 | WOS:000263202500005 |
公开日期 | 2010-12-22 |
源URL | [http://ir.qdio.ac.cn/handle/337002/3071] ![]() |
专题 | 海洋研究所_实验海洋生物学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Key Lab Expt Marin Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China 3.Ocean Univ China, Fac Life Sci & Technol, Coll Marine Life Sci, Qingdao 266003, Peoples R China 4.China Univ Petr, Ctr Biotechnol & Bioengn, Qingdao 266555, Shandong, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Weijie,Guan, Xiangyu,Yang, Yu,et al. Biosynthesis of fluorescent allophycocyanin alpha-subunits by autocatalysis in Escherichia coli[J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,2009,52:135-140. |
APA | Zhang, Weijie.,Guan, Xiangyu.,Yang, Yu.,Ge, Baosheng.,Chen, Huaxin.,...&Qin, Song.(2009).Biosynthesis of fluorescent allophycocyanin alpha-subunits by autocatalysis in Escherichia coli.BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,52,135-140. |
MLA | Zhang, Weijie,et al."Biosynthesis of fluorescent allophycocyanin alpha-subunits by autocatalysis in Escherichia coli".BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 52(2009):135-140. |
入库方式: OAI收割
来源:海洋研究所
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