Cloning and characterization of a novel C-type lectin gene from shrimp Litopenaeus vannamei
文献类型：期刊论文
| 作者 | Zhang, Ying1,2; Qiu, Limei1; Song, Linsheng1; Zhang, Huan1,2; Zhao, Jianmin1; Wang, Lingling1; Yu, Yundong1,2; Li, Chenghua1,2; Li, Fengmei1,3; Xing, Kezhi4 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2009 |
| 卷号 | 26期号:1页码:183-192 |
| 关键词 | C-type Lectin Gene Cloning Recombinant Expression Bacteria Agglutination Litopenaeus Vannamei |
| ISSN号 | 1050-4648 |
| DOI | 10.1016/j.fsi.2008.03.008 |
| 文献子类 | Article |
| 英文摘要 | In invertebrates, C-type lectins play crucial roles in innate immunity responses by mediating the recognition of host cells to pathogens and clearing microinvaders, which interact with carbohydrates and function as pattern recognition receptors (PRRs). A novel C-type lectin gene (LvLec) cDNA was cloned from hemocytes of Litopenaeus vannamei by expressed sequence tag (EST) and rapid amplification of cDNA ends (RACE) PCR. The full-length cDNA of LvLec was of 618 bp, consisting of a 5'-terminal untranslated region (UTR) of 60 bp and a 3'-UTR of 87 bp with a poly (A) tail. The deduced amino acid sequence of LvLec possessed all conserved features critical for the fundamental structure, such as the four cysteine residues (Cys(53), Cys(128), Cys(144), Cys(152)) involved in the formation of disulfides bridges and the potential Ca(2+)/carbohydrate-binding sites. The high similarity and the close phylogenetic relationship of LvLec shared with C-type lectins from vertebrates and invertebrates. The structural features of LvLec indicated that it was an invertebrate counterpart of the C-type lectin family. The cDNA fragment encoding the mature peptide of LvLec was recombined and expressed in Escherichia coli BL21(DE3)-pLysS. The recombinant protein (rLvLec) could agglutinate bacteria E. coli JM109 depending on Ca(2+), and the agglutination could be inhibited by mannose and EDTA. These results indicated that LvLec was a new member of C-type lectin family and involved in the immune defence response to Gram negative bacteria in Litopenaeus vannamei. (C) 2008 Elsevier Ltd. All rights reserved.; In invertebrates, C-type lectins play crucial roles in innate immunity responses by mediating the recognition of host cells to pathogens and clearing microinvaders, which interact with carbohydrates and function as pattern recognition receptors (PRRs). A novel C-type lectin gene (LvLec) cDNA was cloned from hemocytes of Litopenaeus vannamei by expressed sequence tag (EST) and rapid amplification of cDNA ends (RACE) PCR. The full-length cDNA of LvLec was of 618 bp, consisting of a 5'-terminal untranslated region (UTR) of 60 bp and a 3'-UTR of 87 bp with a poly (A) tail. The deduced amino acid sequence of LvLec possessed all conserved features critical for the fundamental structure, such as the four cysteine residues (Cys(53), Cys(128), Cys(144), Cys(152)) involved in the formation of disulfides bridges and the potential Ca2+/carbohydrate-binding sites. The high similarity and the close phylogenetic relationship of LvLec shared with C-type lectins from vertebrates and invertebrates. The structural features of LvLec indicated that it was an invertebrate counterpart of the C-type lectin family. The cDNA fragment encoding the mature peptide of LvLec was recombined and expressed in Escherichia coli BL21(DE3)-pLysS. The recombinant protein (rLvLec) could agglutinate bacteria E. coli JM109 depending on Ca2+, and the agglutination could be inhibited by mannose and EDTA. These results indicated that LvLec was a new member of C-type lectin family and involved in the immune defence response to Gram negative bacteria in Litopenaeus vannamei. (C) 2008 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000264015700023 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3075]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Qingdao Univ Sci & Technol, Qingdao, Peoples R China 4.Tianjin Agr Coll, Tianjin 300381, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Ying,Qiu, Limei,Song, Linsheng,et al. Cloning and characterization of a novel C-type lectin gene from shrimp Litopenaeus vannamei[J]. FISH & SHELLFISH IMMUNOLOGY,2009,26(1):183-192. |
| APA | Zhang, Ying.,Qiu, Limei.,Song, Linsheng.,Zhang, Huan.,Zhao, Jianmin.,...&Huang, Bingxin.(2009).Cloning and characterization of a novel C-type lectin gene from shrimp Litopenaeus vannamei.FISH & SHELLFISH IMMUNOLOGY,26(1),183-192. |
| MLA | Zhang, Ying,et al."Cloning and characterization of a novel C-type lectin gene from shrimp Litopenaeus vannamei".FISH & SHELLFISH IMMUNOLOGY 26.1(2009):183-192. |
入库方式： OAI收割
来源：海洋研究所
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