The preparation and bioactivity research of agaro-oligosaccharides
文献类型：期刊论文
| 作者 | Chen, HM; Zheng, L; Yan, XJ |
| 刊名 | FOOD TECHNOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2005 |
| 卷号 | 43期号:1页码:29-36 |
| 关键词 | Agaro-oligosaccharides Orthogonal Matrix Hplc Acid Degradation Antioxidant Alpha-glucosidase |
| ISSN号 | 1330-9862 |
| 文献子类 | Article |
| 英文摘要 | Agaro-oligosaccharides were hydrolytically obtained from agar using hydrochloric acid, citric acid, and cationic exchange resin (solid acid). The FT-IR and NMR data showed that the hydrolysate has the structure of agaro-oligomers. Orthogonal matrix method was applied to optimize the preparation conditions based on alpha-naphthylamine end-labeled HPLC analysis method. The optimal way for oligosaccharides with different degree of polymerization (DP) was achieved by using solid acid degradation, which could give high yield and avoid solution neutralization process. Agaro-oligosaccharides with high purity were consequently obtained by activated carbon column isolation. Furthermore, the antioxidant and alpha-glucosidase inhibitory activity of three fractions were also investigated. The result indicated that 8% ethanol-eluted fraction showed highest activity against alpha-glucosidase with IC50 of 8.84 mg/mL, while 25% ethanol-eluted fraction possessed excellent antioxidant ability.; Agaro-oligosaccharides were hydrolytically obtained from agar using hydrochloric acid, citric acid, and cationic exchange resin (solid acid). The FT-IR and NMR data showed that the hydrolysate has the structure of agaro-oligomers. Orthogonal matrix method was applied to optimize the preparation conditions based on alpha-naphthylamine end-labeled HPLC analysis method. The optimal way for oligosaccharides with different degree of polymerization (DP) was achieved by using solid acid degradation, which could give high yield and avoid solution neutralization process. Agaro-oligosaccharides with high purity were consequently obtained by activated carbon column isolation. Furthermore, the antioxidant and alpha-glucosidase inhibitory activity of three fractions were also investigated. The result indicated that 8% ethanol-eluted fraction showed highest activity against alpha-glucosidase with IC50 of 8.84 mg/mL, while 25% ethanol-eluted fraction possessed excellent antioxidant ability. |
| 学科主题 | Biotechnology & Applied Microbiology ; Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000227947900004 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3085]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Ningbo Univ, Key Lab Marine Biotechnol, Ningbo 315211, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, HM,Zheng, L,Yan, XJ. The preparation and bioactivity research of agaro-oligosaccharides[J]. FOOD TECHNOLOGY AND BIOTECHNOLOGY,2005,43(1):29-36. |
| APA | Chen, HM,Zheng, L,&Yan, XJ.(2005).The preparation and bioactivity research of agaro-oligosaccharides.FOOD TECHNOLOGY AND BIOTECHNOLOGY,43(1),29-36. |
| MLA | Chen, HM,et al."The preparation and bioactivity research of agaro-oligosaccharides".FOOD TECHNOLOGY AND BIOTECHNOLOGY 43.1(2005):29-36. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。