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Suspension culture of gametophytes of transgenic kelp in a photobioreactor

文献类型：期刊论文

作者Gao, JT; Zhang, YC; Wang, HH; Qin, S
刊名BIOTECHNOLOGY LETTERS
出版日期2005-07-01
卷号27期号:14页码:1025-1028
关键词Bioreactor Gametophytes Macroalga Plasminogen Activator Protein Seaweed Transgenic Kelp
ISSN号0141-5492
DOI10.1007/s10529-005-8098-z
文献子类Article
英文摘要Transgenic Laminaria japonica gametophytes producing a recombinant tissue-type plasminogen activator (rtPA) protein, which is an effective third-generation thrombolytic agent for acute myocardial infarction (AMI), were cultured in an illuminated bubble column bioreactor. A maximum final dry cell weight of 1120 mg l(-1) was obtained in batch culture with an initial dry cell weight of 126 mg l(-1) and with aeration rate of 1.2 l air min(-1) l(-1) culture, nitrate at 1.5 mM and phosphate at 0.17 mM. The yield of rtPA was 56 mu g g(-1) dry cell wt. This is the first report regarding cultivation of a transgenic macroalga in a bioreactor.; Transgenic Laminaria japonica gametophytes producing a recombinant tissue-type plasminogen activator (rtPA) protein, which is an effective third-generation thrombolytic agent for acute myocardial infarction (AMI), were cultured in an illuminated bubble column bioreactor. A maximum final dry cell weight of 1120 mg l(-1) was obtained in batch culture with an initial dry cell weight of 126 mg l(-1) and with aeration rate of 1.2 l air min(-1) l(-1) culture, nitrate at 1.5 mM and phosphate at 0.17 mM. The yield of rtPA was 56 mu g g(-1) dry cell wt. This is the first report regarding cultivation of a transgenic macroalga in a bioreactor.
学科主题Biotechnology & Applied Microbiology
URL标识查看原文
语种英语
WOS记录号WOS:000231471000011
公开日期2010-12-22
源URL[http://ir.qdio.ac.cn/handle/337002/3093]  
专题海洋研究所_实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714		 Gao, JT,Zhang, YC,Wang, HH,et al. Suspension culture of gametophytes of transgenic kelp in a photobioreactor[J]. BIOTECHNOLOGY LETTERS,2005,27(14):1025-1028.
APA Gao, JT,Zhang, YC,Wang, HH,&Qin, S.(2005).Suspension culture of gametophytes of transgenic kelp in a photobioreactor.BIOTECHNOLOGY LETTERS,27(14),1025-1028.
MLA Gao, JT,et al."Suspension culture of gametophytes of transgenic kelp in a photobioreactor".BIOTECHNOLOGY LETTERS 27.14(2005):1025-1028.

入库方式： OAI收割

来源：海洋研究所

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