Scale-up of fermentation and purification of recombinant allophycocyanin over-expressed in Escherichia coli
文献类型：期刊论文
| 作者 | Ge, BS; Tang, ZH; Zhao, FQ; Ren, YH; Yang, Y ; Qin, S
|
| 刊名 | PROCESS BIOCHEMISTRY
 |
| 出版日期 | 2005-10-01 |
| 卷号 | 40期号:10页码:3190-3195 |
| 关键词 | Scale-up Recombinant Allophycocyanin Escherichia Coli Protein Purification Tumour Inhibition |
| ISSN号 | 1359-5113 |
| DOI | 10.1016/j.procbio.2005.02.023 |
| 文献子类 | Article |
| 英文摘要 | Phycobiliprotein is a photosynthetic antenna pigment found in cyanobacteria, rhodophytes, cryptophytes and certain dinoflagellates, which has been found to have anti-oxidative and anti-tumour activities. In this paper, a recombinant allophycocyanin (rAPC) had been expressed in Escherichia coli for anti-tumour effect. E. coli cells were cultured using glucose fed-batch method to achieve high cell densities. The biomass of rAPC was up to 3.52 g/L broth. The rAPC was purified from soluble E. coli cell lysate employing hydrophobic interaction chromatographic (HIC) method developed at the bench scale using 20 mL column. The process was performed at the pilot scale using 500 mL column for evaluation of scale-up. An amylose affinity column was used to improve the purity of final product in pilot scale purification. The purification process resulted in greater than 98% pure product and yielded up to 2.0 g/kg wet cells at the bench scale and 1.2 g/kg wet cells at the pilot scale. Peptide mapping was used to prove the identity of rAPC purified from bench scale and pilot scale process. Purified rAPC at the pilot scale was found to have remarkable inhibition on S-180 carcinoma in mice. (c) 2005 Elsevier Ltd. All rights reserved.; Phycobiliprotein is a photosynthetic antenna pigment found in cyanobacteria, rhodophytes, cryptophytes and certain dinoflagellates, which has been found to have anti-oxidative and anti-tumour activities. In this paper, a recombinant allophycocyanin (rAPC) had been expressed in Escherichia coli for anti-tumour effect. E. coli cells were cultured using glucose fed-batch method to achieve high cell densities. The biomass of rAPC was up to 3.52 g/L broth. The rAPC was purified from soluble E. coli cell lysate employing hydrophobic interaction chromatographic (HIC) method developed at the bench scale using 20 mL column. The process was performed at the pilot scale using 500 mL column for evaluation of scale-up. An amylose affinity column was used to improve the purity of final product in pilot scale purification. The purification process resulted in greater than 98% pure product and yielded up to 2.0 g/kg wet cells at the bench scale and 1.2 g/kg wet cells at the pilot scale. Peptide mapping was used to prove the identity of rAPC purified from bench scale and pilot scale process. Purified rAPC at the pilot scale was found to have remarkable inhibition on S-180 carcinoma in mice. (c) 2005 Elsevier Ltd. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000231833100006 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3095]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ge, BS,Tang, ZH,Zhao, FQ,et al. Scale-up of fermentation and purification of recombinant allophycocyanin over-expressed in Escherichia coli[J]. PROCESS BIOCHEMISTRY,2005,40(10):3190-3195. |
| APA | Ge, BS,Tang, ZH,Zhao, FQ,Ren, YH,Yang, Y,&Qin, S.(2005).Scale-up of fermentation and purification of recombinant allophycocyanin over-expressed in Escherichia coli.PROCESS BIOCHEMISTRY,40(10),3190-3195. |
| MLA | Ge, BS,et al."Scale-up of fermentation and purification of recombinant allophycocyanin over-expressed in Escherichia coli".PROCESS BIOCHEMISTRY 40.10(2005):3190-3195. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。