Cloning and characterization of beta-carotene ketolase gene promoter in Haematococcus pluvialis
文献类型：期刊论文
| 作者 | Meng, CX; Teng, CY; Jiang, P; Qin, S; Tseng, CK |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2005-04-01 |
| 卷号 | 37期号:4页码:270-275 |
| 关键词 | Haematococcus Pluvialis Astaxanthin Beta-carotene Ketolase Gene (Bkt) Cis-acting Element Promoter |
| ISSN号 | 1672-9145 |
| DOI | 10.1111/j.1745-7270.2005.00033.x |
| 文献子类 | Article |
| 英文摘要 | The unicellular green alga Haematococcus pluvialis accumulates a highly valuable ketocarotenoid, astaxanthin, under various environmental stresses. beta-carotene ketolase (BKT) plays a key role in astaxanthin biosynthesis in H. pluvialis. In this paper, an approximate 700 bp 5'-flanking region of the bkt gene containing a putative promoter was cloned through walking upstream. The results of the sequence analysis showed that this bkt 5'-flanking region might have cis-acting elements such as sterol regulatory element (SRE-1)-like motifs, the C-repeat/dehydration responsive element (DRE) and al-3 proximal element (APE)-like motifs, except for typical TATA and CCAAT boxes. The results of the P-galactosidase assay and the transient expression of lacZ driven by a series of sequential deletions revealed that a minimal promoter-like region might exist from -630 to -408 bp, and the highest promoter activity was observed to span the positions from -630 to -308 bp. The results of the site-directed mutagenesis of a C-repeat/DRE and two APE-like motifs in a promoter-like region (-630 to -308 bp) suggested that two APE-like motifs might be essential for transcriptional control of the bkt gene.; The unicellular green alga Haematococcus pluvialis accumulates a highly valuable ketocarotenoid, astaxanthin, under various environmental stresses. beta-carotene ketolase (BKT) plays a key role in astaxanthin biosynthesis in H. pluvialis. In this paper, an approximate 700 bp 5'-flanking region of the bkt gene containing a putative promoter was cloned through walking upstream. The results of the sequence analysis showed that this bkt 5'-flanking region might have cis-acting elements such as sterol regulatory element (SRE-1)-like motifs, the C-repeat/dehydration responsive element (DRE) and al-3 proximal element (APE)-like motifs, except for typical TATA and CCAAT boxes. The results of the P-galactosidase assay and the transient expression of lacZ driven by a series of sequential deletions revealed that a minimal promoter-like region might exist from -630 to -408 bp, and the highest promoter activity was observed to span the positions from -630 to -308 bp. The results of the site-directed mutagenesis of a C-repeat/DRE and two APE-like motifs in a promoter-like region (-630 to -308 bp) suggested that two APE-like motifs might be essential for transcriptional control of the bkt gene. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000228545300009 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3113]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Meng, CX,Teng, CY,Jiang, P,et al. Cloning and characterization of beta-carotene ketolase gene promoter in Haematococcus pluvialis[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2005,37(4):270-275. |
| APA | Meng, CX,Teng, CY,Jiang, P,Qin, S,&Tseng, CK.(2005).Cloning and characterization of beta-carotene ketolase gene promoter in Haematococcus pluvialis.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,37(4),270-275. |
| MLA | Meng, CX,et al."Cloning and characterization of beta-carotene ketolase gene promoter in Haematococcus pluvialis".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 37.4(2005):270-275. |
入库方式： OAI收割
来源：海洋研究所
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