Recombination and heterologous expression of allophycocyanin gene in the chloroplast of Chlamydomonas reinhardtii
文献类型：期刊论文
| 作者 | Su, ZL; Qian, KX; Tan, CP; Meng, CX; Qin, S |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2005-10-01 |
| 卷号 | 37期号:10页码:709-712 |
| 关键词 | Chlamydomonas Reinhardtii Chloroplast Transformation Allophycocyanin Gene |
| ISSN号 | 1672-9145 |
| DOI | 10.1111/j.1745-7270.2005.00092.x |
| 文献子类 | Article |
| 英文摘要 | Heterogeneous expression of multiple genes in the nucleus of transgenic plants requires the introduction of an individual gene and the subsequent backcross to reconstitute multi-subunit proteins or metabolic pathways. In order to accomplish the expression of multiple genes in a single transformation event, we inserted both large and small subunits of allophycocyanin gene (apcA and apcB) into Chlamydomonas reinhardtii chloroplast expression vector, resulting in papc-S. The constructed vector was then introduced into the chloroplast of C. reinhardtii by micro-particle bombardment. Polymerase chain reaction and Southern blot analysis revealed that the two genes had integrated into the chloroplast genome. Western blot and enzyme-linked immunosorbent assay showed that the two genes from the prokaryotic cyanobacteria could be correctly expressed in the chloroplasts of C. reinhardtii. The expressed foreign protein in transformants accounted for about 2%-3% of total soluble proteins. These findings pave the way to the reconstitution of multi-subunit proteins or metabolic pathways in transgenic C. reinhardtii chloroplasts in a single transformation event.; Heterogeneous expression of multiple genes in the nucleus of transgenic plants requires the introduction of an individual gene and the subsequent backcross to reconstitute multi-subunit proteins or metabolic pathways. In order to accomplish the expression of multiple genes in a single transformation event, we inserted both large and small subunits of allophycocyanin gene (apcA and apcB) into Chlamydomonas reinhardtii chloroplast expression vector, resulting in papc-S. The constructed vector was then introduced into the chloroplast of C. reinhardtii by micro-particle bombardment. Polymerase chain reaction and Southern blot analysis revealed that the two genes had integrated into the chloroplast genome. Western blot and enzyme-linked immunosorbent assay showed that the two genes from the prokaryotic cyanobacteria could be correctly expressed in the chloroplasts of C. reinhardtii. The expressed foreign protein in transformants accounted for about 2%-3% of total soluble proteins. These findings pave the way to the reconstitution of multi-subunit proteins or metabolic pathways in transgenic C. reinhardtii chloroplasts in a single transformation event. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000232675000009 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3129]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Zhejiang Univ, Dept Biotechnol, Coll Life Sci, Hangzhou 310027, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 3.Qingdao Univ Sci & Technol, Qingdao 266061, Peoples R China 4.Grad Univ, Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Su, ZL,Qian, KX,Tan, CP,et al. Recombination and heterologous expression of allophycocyanin gene in the chloroplast of Chlamydomonas reinhardtii[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2005,37(10):709-712. |
| APA | Su, ZL,Qian, KX,Tan, CP,Meng, CX,&Qin, S.(2005).Recombination and heterologous expression of allophycocyanin gene in the chloroplast of Chlamydomonas reinhardtii.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,37(10),709-712. |
| MLA | Su, ZL,et al."Recombination and heterologous expression of allophycocyanin gene in the chloroplast of Chlamydomonas reinhardtii".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 37.10(2005):709-712. |
入库方式： OAI收割
来源：海洋研究所
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