Establishment of a micro-particle bombardment transformation system for Dunaliella saliva
文献类型：期刊论文
| 作者 | Tan, CP; Qin, S; Zhang, Q ; Jiang, P; Zhao, FQ
|
| 刊名 | JOURNAL OF MICROBIOLOGY
 |
| 出版日期 | 2005-08-01 |
| 卷号 | 43期号:4页码:361-365 |
| 关键词 | Dunaliella Salina Micro-particle Bombardment Gus Bar |
| ISSN号 | 1225-8873 |
| 文献子类 | Article |
| 英文摘要 | In this study, we chronicle the establishment of a novel transformation system for the unicellular marine green alga, Dunaliella salina. We introduced the CaMV35S promoter-GUS construct into D. saliva with a PDS1000/He micro-particle bombardment system. Forty eight h after transformation, via histochemical staining, we observed the transient expression of GUS in D. salina cells which had been bombarded under rupture-disc pressures of 450 psi and 900 psi. We observed no GUS activity in either the negative or the blank controls. Our findings indicated that the micro-particle bombardment method constituted a feasible approach to the genetic transformation of D. salina. We also conducted tests of the cells' sensitivity to seven antibiotics and one herbicide, and our results suggested that 20 mu g/ ml of Basta could inhibit cell growth completely. The bar gene, which encodes for phosphinothricin acetyltransferase and confers herbicide tolerance, was introduced into the cells via the above established method. The results of PCR and PCR-Southern blot analyses indicated that the gene was successfully integrated into the genome of the transformants.; In this study, we chronicle the establishment of a novel transformation system for the unicellular marine green alga, Dunaliella salina. We introduced the CaMV35S promoter-GUS construct into D. saliva with a PDS1000/He micro-particle bombardment system. Forty eight h after transformation, via histochemical staining, we observed the transient expression of GUS in D. salina cells which had been bombarded under rupture-disc pressures of 450 psi and 900 psi. We observed no GUS activity in either the negative or the blank controls. Our findings indicated that the micro-particle bombardment method constituted a feasible approach to the genetic transformation of D. salina. We also conducted tests of the cells' sensitivity to seven antibiotics and one herbicide, and our results suggested that 20 mu g/ ml of Basta could inhibit cell growth completely. The bar gene, which encodes for phosphinothricin acetyltransferase and confers herbicide tolerance, was introduced into the cells via the above established method. The results of PCR and PCR-Southern blot analyses indicated that the gene was successfully integrated into the genome of the transformants. |
| 学科主题 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000231609900007 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3131]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Acad Sinica, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tan, CP,Qin, S,Zhang, Q,et al. Establishment of a micro-particle bombardment transformation system for Dunaliella saliva[J]. JOURNAL OF MICROBIOLOGY,2005,43(4):361-365. |
| APA | Tan, CP,Qin, S,Zhang, Q,Jiang, P,&Zhao, FQ.(2005).Establishment of a micro-particle bombardment transformation system for Dunaliella saliva.JOURNAL OF MICROBIOLOGY,43(4),361-365. |
| MLA | Tan, CP,et al."Establishment of a micro-particle bombardment transformation system for Dunaliella saliva".JOURNAL OF MICROBIOLOGY 43.4(2005):361-365. |
入库方式： OAI收割
来源：海洋研究所
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