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Identification of 27 Porphyra lines (Rhodophyta) by DNA fingerprinting and molecular markers

文献类型：期刊论文

作者Weng, ML; Liu, B; Jin, DM; Yang, QK; Zhao, G; Ma, JH; Xu, P; Duan, DL; Wang, B
刊名JOURNAL OF APPLIED PHYCOLOGY
出版日期2005
卷号17期号:1页码:91-97
关键词Dna Fingerprinting Porphyra Random Amplified Polymorphic Dna (Rapd) Sequence Characterized Amplification Region (Scar) Sequence Tagged Site (Sts) Specific Molecular Markers
ISSN号0921-8971
DOI10.1007/s10811-005-4845-8
文献子类Article
英文摘要Twenty-seven Porphyra lines, including lines widely used in China, wild lines and lines introduced to China from abroad in recent years, were screened by random amplified polymorphic DNA (RAPD) technique with 120 operon primers. From the generated RAPD products, 11 bands that showed stable and repeatable RAPD patterns amplified by OPC-04, OPJ-18 and OPX-06, respectively were scored and used to develop the DNA fingerprints of the 27 Porphyra lines. Moreover, the DNA fingerprinting patterns were converted into computer language expressed with two digitals, 1 and 0, which represented the presence (numbered as 1) or absence (numbered as 0) of the corresponding band, respectively. Based on the above results, computerized DNA fingerprints were constructed in which each of the 27 Porphyra lines has its unique fingerprinting pattern and can be easily distinguished from others. Software named PGI (Porphyra germplasm identification) was designed for identification of the 27 Porphyra lines. In addition, seven specific RAPD markers from seven Porphyra lines were identified and two of them were successfully converted into SCAR (sequence characterized amplification region) markers. The developed DNA fingerprinting and specific molecular markers provide useful ways for the identification, classification and resource protection of the Porphyra lines.; Twenty-seven Porphyra lines, including lines widely used in China, wild lines and lines introduced to China from abroad in recent years, were screened by random amplified polymorphic DNA (RAPD) technique with 120 operon primers. From the generated RAPD products, 11 bands that showed stable and repeatable RAPD patterns amplified by OPC-04, OPJ-18 and OPX-06, respectively were scored and used to develop the DNA fingerprints of the 27 Porphyra lines. Moreover, the DNA fingerprinting patterns were converted into computer language expressed with two digitals, 1 and 0, which represented the presence (numbered as 1) or absence (numbered as 0) of the corresponding band, respectively. Based on the above results, computerized DNA fingerprints were constructed in which each of the 27 Porphyra lines has its unique fingerprinting pattern and can be easily distinguished from others. Software named PGI (Porphyra germplasm identification) was designed for identification of the 27 Porphyra lines. In addition, seven specific RAPD markers from seven Porphyra lines were identified and two of them were successfully converted into SCAR (sequence characterized amplification region) markers. The developed DNA fingerprinting and specific molecular markers provide useful ways for the identification, classification and resource protection of the Porphyra lines.
学科主题Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
URL标识查看原文
语种英语
WOS记录号WOS:000229904700014
公开日期2010-12-22
源URL[http://ir.qdio.ac.cn/handle/337002/3143]  
专题海洋研究所_实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
2.Shanghai Fisheries Univ, Shanghai 200090, Peoples R China
3.Jiangsu Inst Oceanol & Hydrol, Nantong 226007, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714		 Weng, ML,Liu, B,Jin, DM,et al. Identification of 27 Porphyra lines (Rhodophyta) by DNA fingerprinting and molecular markers[J]. JOURNAL OF APPLIED PHYCOLOGY,2005,17(1):91-97.
APA Weng, ML.,Liu, B.,Jin, DM.,Yang, QK.,Zhao, G.,...&Wang, B.(2005).Identification of 27 Porphyra lines (Rhodophyta) by DNA fingerprinting and molecular markers.JOURNAL OF APPLIED PHYCOLOGY,17(1),91-97.
MLA Weng, ML,et al."Identification of 27 Porphyra lines (Rhodophyta) by DNA fingerprinting and molecular markers".JOURNAL OF APPLIED PHYCOLOGY 17.1(2005):91-97.

入库方式： OAI收割

来源：海洋研究所

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