Toxicity and protective efficiency of cryoprotectants to flounder (Paralichthys olivaceus) embryos
文献类型：期刊论文
| 作者 | Zhang, YZ; Zhang, SC; Liu, XZ; Xu, YJ ; Hu, JH; Xu, YY; Li, J; Chen, SL
|
| 刊名 | THERIOGENOLOGY
 |
| 出版日期 | 2005-02-01 |
| 卷号 | 63期号:3页码:763-773 |
| 关键词 | Flounder Embryo Cryoprotectant Toxicity Protective Efficiency |
| ISSN号 | 0093-691X |
| DOI | 10.1016/j.theriogenology.2004.04.011 |
| 文献子类 | Article |
| 英文摘要 | With the purpose of finding an ideal cryoprotectant or combination of cryoprotectants in a suitable concentration for flounder (Paralichthys olivaceus) embryo cryopreservation, we tested the toxicities, at culture temperature (16 degrees C), of five most commonly used cryoprotectants-dimethyl sulfoxide (Me2SO), glycerol, methanol (MeOH), 1,2-propylene glycol (PG) and ethylene glycol (EG). In addition, cryoprotective efficiency to flounder embryos of individual and combined cryoprotectants were tested at -15 degrees C for 60 min. Five different concentrations of each of the five cryoprotectants and 20 different combinations of these cryoprotectants were tested for their protective efficiency. The results showed that the toxicity to flounder embryos of the five cryoprotectants are in the following sequence: PG < MeOH < Me2SO < glycerol < EG (P < 0.05); whereas the protective efficiency of each cryoprotectant, at -15 degrees C for a period of 60 min, are in the following sequence: PG > Me2SO approximate to MeOH approximate to glycerol > EG (greater symbols mean P < 0.05, and approximate symbols mean P > 0.05). Methanol combined with any one of the other cryoprotectants gave the best protection, while ethylene glycol combined with any one of the other cryoprotectants gave the poorest protection at -15 degrees C. Toxicity effect was concentration dependent with the lowest concentration being the least toxic for all five cryoprotectants at 16 degrees C. For PG, MeOH and glycerol, 20% solutions gave the best protection at -15 degrees C; whereas a 15% solution of Me2SO, and a 10% solution of EG, gave the best protection at -15 degrees C. (c) 2004 Elsevier Inc. All rights reserved.; With the purpose of finding an ideal cryoprotectant or combination of cryoprotectants in a suitable concentration for flounder (Paralichthys olivaceus) embryo cryopreservation, we tested the toxicities, at culture temperature (16 degrees C), of five most commonly used cryoprotectants-dimethyl sulfoxide (Me2SO), glycerol, methanol (MeOH), 1,2-propylene glycol (PG) and ethylene glycol (EG). In addition, cryoprotective efficiency to flounder embryos of individual and combined cryoprotectants were tested at -15 degrees C for 60 min. Five different concentrations of each of the five cryoprotectants and 20 different combinations of these cryoprotectants were tested for their protective efficiency. The results showed that the toxicity to flounder embryos of the five cryoprotectants are in the following sequence: PG < MeOH < Me2SO < glycerol < EG (P < 0.05); whereas the protective efficiency of each cryoprotectant, at -15 degrees C for a period of 60 min, are in the following sequence: PG > Me2SO approximate to MeOH approximate to glycerol > EG (greater symbols mean P < 0.05, and approximate symbols mean P > 0.05). Methanol combined with any one of the other cryoprotectants gave the best protection, while ethylene glycol combined with any one of the other cryoprotectants gave the poorest protection at -15 degrees C. Toxicity effect was concentration dependent with the lowest concentration being the least toxic for all five cryoprotectants at 16 degrees C. For PG, MeOH and glycerol, 20% solutions gave the best protection at -15 degrees C; whereas a 15% solution of Me2SO, and a 10% solution of EG, gave the best protection at -15 degrees C. (c) 2004 Elsevier Inc. All rights reserved. |
| 学科主题 | Reproductive Biology ; Veterinary Sciences |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000227660400007 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3169]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Ocean Univ Qingdao, Dept Marine Biol, Qingdao 266003, Peoples R China 2.Liaocheng Univ, Dept Life Sci, Liaocheng 252059, Peoples R China 3.Chinese Acad Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, YZ,Zhang, SC,Liu, XZ,et al. Toxicity and protective efficiency of cryoprotectants to flounder (Paralichthys olivaceus) embryos[J]. THERIOGENOLOGY,2005,63(3):763-773. |
| APA | Zhang, YZ.,Zhang, SC.,Liu, XZ.,Xu, YJ.,Hu, JH.,...&Chen, SL.(2005).Toxicity and protective efficiency of cryoprotectants to flounder (Paralichthys olivaceus) embryos.THERIOGENOLOGY,63(3),763-773. |
| MLA | Zhang, YZ,et al."Toxicity and protective efficiency of cryoprotectants to flounder (Paralichthys olivaceus) embryos".THERIOGENOLOGY 63.3(2005):763-773. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。