A new method for screening alpha-glucosidase inhibitors and application to marine microorganisms
文献类型：期刊论文
| 作者 | Chen, HM; Yan, XJ; Lin, W; Zheng, L; Zhang, WW |
| 刊名 | PHARMACEUTICAL BIOLOGY
 |
| 出版日期 | 2004-09-01 |
| 卷号 | 42期号:6页码:416-421 |
| 关键词 | Enzyme Assay Alpha-glucosidase Inhibitor Marine Microorganism |
| ISSN号 | 1388-0209 |
| DOI | 10.1080/13880200490885987 |
| 文献子类 | Article |
| 英文摘要 | A new enzyme assay method for screening alpha-glucosidase inhibitors with rapidity and simplicity was developed. The enzyme-substituted alpha-glucosidases for this assay was glucoamylase. Samples were spotted or developed on the silica gel plate. The agar solution containing substrate was poured on the plate, and paper impregnated with enzyme was layered on the agar. After incubation, an inhibitory circle would appear around the inhibitor. By using this method, more than 200 strains of marine microorganisms were screened. Among them, three active strains were found to secrete inhibitors in the culture medium.; A new enzyme assay method for screening alpha-glucosidase inhibitors with rapidity and simplicity was developed. The enzyme-substituted alpha-glucosidases for this assay was glucoamylase. Samples were spotted or developed on the silica gel plate. The agar solution containing substrate was poured on the plate, and paper impregnated with enzyme was layered on the agar. After incubation, an inhibitory circle would appear around the inhibitor. By using this method, more than 200 strains of marine microorganisms were screened. Among them, three active strains were found to secrete inhibitors in the culture medium. |
| 学科主题 | Plant Sciences ; Medical Laboratory Technology ; Pharmacology & Pharmacy |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000226444900006 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3179]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Ningbo Univ, Key Lab Marine Biotechnol, Ningbo 315211, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, HM,Yan, XJ,Lin, W,et al. A new method for screening alpha-glucosidase inhibitors and application to marine microorganisms[J]. PHARMACEUTICAL BIOLOGY,2004,42(6):416-421. |
| APA | Chen, HM,Yan, XJ,Lin, W,Zheng, L,&Zhang, WW.(2004).A new method for screening alpha-glucosidase inhibitors and application to marine microorganisms.PHARMACEUTICAL BIOLOGY,42(6),416-421. |
| MLA | Chen, HM,et al."A new method for screening alpha-glucosidase inhibitors and application to marine microorganisms".PHARMACEUTICAL BIOLOGY 42.6(2004):416-421. |
入库方式： OAI收割
来源：海洋研究所
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