Product monitoring and quantitation of oligosaccharides composition in agar hydrolysates by precolumn labeling HPLC
文献类型：期刊论文
| 作者 | Chen, HM; Zheng, L; Lin, W; Yan, XJ |
| 刊名 | TALANTA
 |
| 出版日期 | 2004-10-20 |
| 卷号 | 64期号:3页码:773-777 |
| 关键词 | Agarobiose Oligomers Hplc Alpha-naphthylamine Agaro-oligosaccharides |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2004.04.002 |
| 文献子类 | Article |
| 英文摘要 | Since the discovery of multiple bioactivities for agarobiose oligomers, a quantitative method has been in great need to monitor the agarobiose oligomers. This report demonstrates that agarobiose oligomers can be separated with high resolution in HPLC after introducing a-naphthylamine into compounds. Agarobiose oligomers ranged from biose to decaose were isolated by Sephadex column. HPLC analysis indicated that each oliomer could be quantified with good linearity and a low detection limit of 0.1-4 mug/ml. The chromatographic profiles of agaro-oligosaccharides with different hydrolysis modes (hydrochloride, citric acid, solid acid, and hydroxyl radical degradation) showed that agarobiose could be obtained more than 57.8% using solid acid mediated hydrolysis, while hydrochloride acid could degrade agar into a series of agaro-oligosaccharides from biose to decaose. The yield of oligosaccharides was low if hydrolyzed by citric acid. The Fenton degradation can increase the speed of hydrolysis, but the product was complex. (C) 2004 Elsevier B.V. All rights reserved.; Since the discovery of multiple bioactivities for agarobiose oligomers, a quantitative method has been in great need to monitor the agarobiose oligomers. This report demonstrates that agarobiose oligomers can be separated with high resolution in HPLC after introducing a-naphthylamine into compounds. Agarobiose oligomers ranged from biose to decaose were isolated by Sephadex column. HPLC analysis indicated that each oliomer could be quantified with good linearity and a low detection limit of 0.1-4 mug/ml. The chromatographic profiles of agaro-oligosaccharides with different hydrolysis modes (hydrochloride, citric acid, solid acid, and hydroxyl radical degradation) showed that agarobiose could be obtained more than 57.8% using solid acid mediated hydrolysis, while hydrochloride acid could degrade agar into a series of agaro-oligosaccharides from biose to decaose. The yield of oligosaccharides was low if hydrolyzed by citric acid. The Fenton degradation can increase the speed of hydrolysis, but the product was complex. (C) 2004 Elsevier B.V. All rights reserved. |
| 学科主题 | Chemistry, Analytical |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000224285300029 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3185]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Ningbo Univ, Key Lab Marine Biotechnol, Ningbo 315211, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, HM,Zheng, L,Lin, W,et al. Product monitoring and quantitation of oligosaccharides composition in agar hydrolysates by precolumn labeling HPLC[J]. TALANTA,2004,64(3):773-777. |
| APA | Chen, HM,Zheng, L,Lin, W,&Yan, XJ.(2004).Product monitoring and quantitation of oligosaccharides composition in agar hydrolysates by precolumn labeling HPLC.TALANTA,64(3),773-777. |
| MLA | Chen, HM,et al."Product monitoring and quantitation of oligosaccharides composition in agar hydrolysates by precolumn labeling HPLC".TALANTA 64.3(2004):773-777. |
入库方式： OAI收割
来源：海洋研究所
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