An efficient method for DNA isolation from red algae
文献类型：期刊论文
| 作者 | Hu, ZN; Zeng, XQ; Wang, AH; Shi, CJ; Duan, DL |
| 刊名 | JOURNAL OF APPLIED PHYCOLOGY
 |
| 出版日期 | 2004-06-01 |
| 卷号 | 16期号:3页码:161-166 |
| 关键词 | Dna Isolation Genetic Manipulation Pcr Amplification Polysaccharides Red Algae |
| ISSN号 | 0921-8971 |
| 文献子类 | Article |
| 英文摘要 | A simple, inexpensive and efficient method was developed for rapid isolation of total genomic DNA from 15 red algal species. It resulted in 0.1 mug high quality DNA from 1 mg fresh algal material, with an A(260)/A(280) ratio of 1.68 - 1.90. Using this rapidly isolated DNA, the 18S ribosomal RNA genes ( rDNA) and the nuclear ribosomal DNA of the internal transcribed spacer (ITS) regions were amplified. The tested DNA was suitable for restriction endonuclease digestion, genetic marker analysis and polymerase chain reaction (PCR) amplification, and may be valid for other genetic manipulation.; A simple, inexpensive and efficient method was developed for rapid isolation of total genomic DNA from 15 red algal species. It resulted in 0.1 mug high quality DNA from 1 mg fresh algal material, with an A(260)/A(280) ratio of 1.68 - 1.90. Using this rapidly isolated DNA, the 18S ribosomal RNA genes ( rDNA) and the nuclear ribosomal DNA of the internal transcribed spacer (ITS) regions were amplified. The tested DNA was suitable for restriction endonuclease digestion, genetic marker analysis and polymerase chain reaction (PCR) amplification, and may be valid for other genetic manipulation. |
| 学科主题 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000225180200001 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3197]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Ocean Univ Qingdao, Coll Fisheries, Qingdao 266003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, ZN,Zeng, XQ,Wang, AH,et al. An efficient method for DNA isolation from red algae[J]. JOURNAL OF APPLIED PHYCOLOGY,2004,16(3):161-166. |
| APA | Hu, ZN,Zeng, XQ,Wang, AH,Shi, CJ,&Duan, DL.(2004).An efficient method for DNA isolation from red algae.JOURNAL OF APPLIED PHYCOLOGY,16(3),161-166. |
| MLA | Hu, ZN,et al."An efficient method for DNA isolation from red algae".JOURNAL OF APPLIED PHYCOLOGY 16.3(2004):161-166. |
入库方式： OAI收割
来源：海洋研究所
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