Genomic cloning, expression and recombinant protein purification of alpha and beta subunits of the allophycocyanin gene (apc) from the cyanobacterium Anacystis nidulans UTEX 625
文献类型：期刊论文
| 作者 | Qin, S; Tang, ZH; Lin, F; Sung, LPA; Tseng, CK |
| 刊名 | JOURNAL OF APPLIED PHYCOLOGY
 |
| 出版日期 | 2004-12-01 |
| 卷号 | 16期号:6页码:483-487 |
| 关键词 | Allophycocyanin Gene Anacystis Nidulans Cyanobacterium Synechococcus Pcc 6301 Recombinant Protein |
| ISSN号 | 0921-8971 |
| DOI | 10.1007/s10811-005-5561-0 |
| 文献子类 | Article |
| 英文摘要 | A genomic fragment encoding alpha(APC) and beta(APC) (i.e., alpha and beta units of the allophycocyanin, APC) from Anacystis nidulans UTEX 625 was cloned and sequenced. This fragment, containing a non-coding sequence of 56 nucleotides in between, was then subcloned into the expression vector pMal-c2 downstream from and in frame with the malE gene of E. coli encoding MBP ( maltose binding protein). The fusion protein was purified by amylose affinity chromatography and cleaved by coagulation factor Xa. alpha(APC) and beta(APC) were then separated from MBP and MBP fusion proteins, respectively, and concentrated by membrane centrifugation. The study provides a method to produce recombinant allophycocyanin subunits for biomedical and biotechnological applications.; A genomic fragment encoding alpha(APC) and beta(APC) (i.e., alpha and beta units of the allophycocyanin, APC) from Anacystis nidulans UTEX 625 was cloned and sequenced. This fragment, containing a non-coding sequence of 56 nucleotides in between, was then subcloned into the expression vector pMal-c2 downstream from and in frame with the malE gene of E. coli encoding MBP ( maltose binding protein). The fusion protein was purified by amylose affinity chromatography and cleaved by coagulation factor Xa. alpha(APC) and beta(APC) were then separated from MBP and MBP fusion proteins, respectively, and concentrated by membrane centrifugation. The study provides a method to produce recombinant allophycocyanin subunits for biomedical and biotechnological applications. |
| 学科主题 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000230724000010 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3213]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 2.Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA |
| 推荐引用方式 GB/T 7714 | Qin, S,Tang, ZH,Lin, F,et al. Genomic cloning, expression and recombinant protein purification of alpha and beta subunits of the allophycocyanin gene (apc) from the cyanobacterium Anacystis nidulans UTEX 625[J]. JOURNAL OF APPLIED PHYCOLOGY,2004,16(6):483-487. |
| APA | Qin, S,Tang, ZH,Lin, F,Sung, LPA,&Tseng, CK.(2004).Genomic cloning, expression and recombinant protein purification of alpha and beta subunits of the allophycocyanin gene (apc) from the cyanobacterium Anacystis nidulans UTEX 625.JOURNAL OF APPLIED PHYCOLOGY,16(6),483-487. |
| MLA | Qin, S,et al."Genomic cloning, expression and recombinant protein purification of alpha and beta subunits of the allophycocyanin gene (apc) from the cyanobacterium Anacystis nidulans UTEX 625".JOURNAL OF APPLIED PHYCOLOGY 16.6(2004):483-487. |
入库方式： OAI收割
来源：海洋研究所
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