DNA fingerprinting of selected Laminaria (Phaeophyta) gametophytes by RAPD markers
文献类型：期刊论文
| 作者 | Wang, XL ; Yang, YX; Cong, YZ; Duan, DL
|
| 刊名 | AQUACULTURE
 |
| 出版日期 | 2004-09-01 |
| 卷号 | 238期号:1-4页码:143-153 |
| 关键词 | Laminaria Gametophytes Germplasm Rapd Scar Fingerprint |
| ISSN号 | 0044-8486 |
| DOI | 10.1016/j.aquaculture.2004.05.007 |
| 文献子类 | Article |
| 英文摘要 | Random amplified polymorphism DNA (RAPD) analysis was applied to germplasm characterization in 33 different selected Laminaria male and female gametophytes. The positional homology of the RAPD analysis using sequence characterized applied region (SCAR) method was successfully conducted. A total of 233 polymorphic loci were obtained from 18 selected primers after screening, of which 27 stable and clear bands were selected to construct a fingerprint map for discrimination of each gametophyte. Seven RAPD markers from five primers were finally determined by a computer program to construct the fingerprint map. Three specific markers closely related with gametophytes were obtained and were converted to gametophytic SCAR markers, the first SCAR marker report on Laminaria germplasm and applicable to cultivars identification. These results demonstrated the feasibility of applying RAPD markers to germplasm characterization in selected Laminaria gametophytes, and can provide a molecular basis for breeding new Laminaria strains. (C) 2004 Elsevier B.V. All rights reserved.; Random amplified polymorphism DNA (RAPD) analysis was applied to germplasm characterization in 33 different selected Laminaria male and female gametophytes. The positional homology of the RAPD analysis using sequence characterized applied region (SCAR) method was successfully conducted. A total of 233 polymorphic loci were obtained from 18 selected primers after screening, of which 27 stable and clear bands were selected to construct a fingerprint map for discrimination of each gametophyte. Seven RAPD markers from five primers were finally determined by a computer program to construct the fingerprint map. Three specific markers closely related with gametophytes were obtained and were converted to gametophytic SCAR markers, the first SCAR marker report on Laminaria germplasm and applicable to cultivars identification. These results demonstrated the feasibility of applying RAPD markers to germplasm characterization in selected Laminaria gametophytes, and can provide a molecular basis for breeding new Laminaria strains. (C) 2004 Elsevier B.V. All rights reserved. |
| 学科主题 | Fisheries ; Marine & Freshwater Biology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000224004300012 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3229]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 海洋研究所_海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Expt Marine Biol Lab, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100081, Peoples R China 3.Shandong Oriental Ocean Sci Tech Co Ltd, Yantai 265618, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, XL,Yang, YX,Cong, YZ,et al. DNA fingerprinting of selected Laminaria (Phaeophyta) gametophytes by RAPD markers[J]. AQUACULTURE,2004,238(1-4):143-153. |
| APA | Wang, XL,Yang, YX,Cong, YZ,&Duan, DL.(2004).DNA fingerprinting of selected Laminaria (Phaeophyta) gametophytes by RAPD markers.AQUACULTURE,238(1-4),143-153. |
| MLA | Wang, XL,et al."DNA fingerprinting of selected Laminaria (Phaeophyta) gametophytes by RAPD markers".AQUACULTURE 238.1-4(2004):143-153. |
入库方式： OAI收割
来源：海洋研究所
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