Programmed cell death in Laminaria japonica (Phaeophyta) tissues infected with alginic acid decomposing bacterium
文献类型：期刊论文
| 作者 | Wang, GG; Lin, W; Zhang, LJ; Yan, XJ; Duan, DL |
| 刊名 | PROGRESS IN NATURAL SCIENCE
 |
| 出版日期 | 2004-12-01 |
| 卷号 | 14期号:12页码:1064-+ |
| 关键词 | Alginic Acid Decomposing Bacterium Caspase-3 Laminaria Japonica Hypersensitive Reaction (Hr) Programmed Cell Death (Pcd) Tunel |
| ISSN号 | 1002-0071 |
| 文献子类 | Article |
| 英文摘要 | TdT-mediated dUTP-biotin nick end labeling (TUNEL) is a sensitive and valid method for detecting DNA cleavage in programmed cell death (PCD). Using this method, DNA cleavage was observed in Laminaria japonica sporophytic tissues, which were infected with alginic acid decomposing bacterium. It was found that DNA cleavage occurred 5 min after the infection, the fragments with 3'-OH groups of cleaved nuclear DNA increased with time of infection and spread from the infection site. Although no typical DNA ladder (200 bp/ 180 bp) was detected by routine agarose gel electrophoresis, the cleavage of nuclear DNA fragments of 97 similar to 48.5 kb could be detected by pulsed field gel electrophoresis (PFGE). By using CaspGLOW(TM) fluorescein active caspase-3 staining method, caspase-3 activity has been detected in response to the infection of alginic acid decomposing bacterium. Our results are similar to the observations in hypersensitive response (HR) of higher plant, suggesting that the rapid cell death of L. japonica infected by alginic acid decomposing bacterium might be involved in PCD, and indicating that the occurrence of PCD is an active defense process against the pathogen's infection.; TdT-mediated dUTP-biotin nick end labeling (TUNEL) is a sensitive and valid method for detecting DNA cleavage in programmed cell death (PCD). Using this method, DNA cleavage was observed in Laminaria japonica sporophytic tissues, which were infected with alginic acid decomposing bacterium. It was found that DNA cleavage occurred 5 min after the infection, the fragments with 3'-OH groups of cleaved nuclear DNA increased with time of infection and spread from the infection site. Although no typical DNA ladder (200 bp/ 180 bp) was detected by routine agarose gel electrophoresis, the cleavage of nuclear DNA fragments of 97 similar to 48.5 kb could be detected by pulsed field gel electrophoresis (PFGE). By using CaspGLOW(TM) fluorescein active caspase-3 staining method, caspase-3 activity has been detected in response to the infection of alginic acid decomposing bacterium. Our results are similar to the observations in hypersensitive response (HR) of higher plant, suggesting that the rapid cell death of L. japonica infected by alginic acid decomposing bacterium might be involved in PCD, and indicating that the occurrence of PCD is an active defense process against the pathogen's infection. |
| 学科主题 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000225781100005 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3231]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, GG,Lin, W,Zhang, LJ,et al. Programmed cell death in Laminaria japonica (Phaeophyta) tissues infected with alginic acid decomposing bacterium[J]. PROGRESS IN NATURAL SCIENCE,2004,14(12):1064-+. |
| APA | Wang, GG,Lin, W,Zhang, LJ,Yan, XJ,&Duan, DL.(2004).Programmed cell death in Laminaria japonica (Phaeophyta) tissues infected with alginic acid decomposing bacterium.PROGRESS IN NATURAL SCIENCE,14(12),1064-+. |
| MLA | Wang, GG,et al."Programmed cell death in Laminaria japonica (Phaeophyta) tissues infected with alginic acid decomposing bacterium".PROGRESS IN NATURAL SCIENCE 14.12(2004):1064-+. |
入库方式： OAI收割
来源：海洋研究所
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