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Optimization of triploid induction by heat shock in Chinese shrimp Fenneropenaeus chinensis

文献类型：期刊论文

作者Li, FH; Xiang, JH; Zhou, LH; Wu, CG; Zhang, XJ
刊名AQUACULTURE
出版日期2003-04-02
卷号219期号:1-4页码:221-231
关键词Triploid Heat Shock Chinese Shrimp Fenneropenaeus Chinensis
ISSN号0044-8486
DOI10.1016/S0044-8486(03)00006-1
文献子类Article
英文摘要Chromosome manipulation for commercially valuable marine animals plays an important role in aquaculture. The special reproductive characteristics of shrimp make it difficult to control fertilization and synchronize egg development, so research on chromosome manipulation in shrimp has proceeded very slowly. In the present study, triploid shrimp Fenneropenaeus chinensis were induced by heat shocks and the optimal-inducing condition was screened at different spawning temperatures. Level of triploid induction for each treatment was evaluated by flow cytometry at nauplius stage. The highest level of triploid induction reached to more than 90%. Starting time for each treatment was very crucial for triploid induction in shrimp. One optimal treatment condition for triploid induction was heat shock (29-32 degreesC), starting at 18-20 min for duration of 10 min. These conditions varied depending on the temperature at spawning. Triploid level at embryo stage and nauplius stage was not different, suggesting the same hatching rate between diploids and triploids. Heat shock is a very effective way to induce triploids in this species, and can be easily used on large scale without any harmful effect on the environment as compared with chemical treatment. (C) 2003 Elsevier Science B.V. All rights reserved.; Chromosome manipulation for commercially valuable marine animals plays an important role in aquaculture. The special reproductive characteristics of shrimp make it difficult to control fertilization and synchronize egg development, so research on chromosome manipulation in shrimp has proceeded very slowly. In the present study, triploid shrimp Fenneropenaeus chinensis were induced by heat shocks and the optimal-inducing condition was screened at different spawning temperatures. Level of triploid induction for each treatment was evaluated by flow cytometry at nauplius stage. The highest level of triploid induction reached to more than 90%. Starting time for each treatment was very crucial for triploid induction in shrimp. One optimal treatment condition for triploid induction was heat shock (29-32 degreesC), starting at 18-20 min for duration of 10 min. These conditions varied depending on the temperature at spawning. Triploid level at embryo stage and nauplius stage was not different, suggesting the same hatching rate between diploids and triploids. Heat shock is a very effective way to induce triploids in this species, and can be easily used on large scale without any harmful effect on the environment as compared with chemical treatment. (C) 2003 Elsevier Science B.V. All rights reserved.
学科主题Fisheries ; Marine & Freshwater Biology
URL标识查看原文
语种英语
WOS记录号WOS:000181802700017
公开日期2010-12-22
源URL[http://ir.qdio.ac.cn/handle/337002/3255]  
专题海洋研究所_实验海洋生物学重点实验室
海洋研究所_海洋生态与环境科学重点实验室
作者单位Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714		 Li, FH,Xiang, JH,Zhou, LH,et al. Optimization of triploid induction by heat shock in Chinese shrimp Fenneropenaeus chinensis[J]. AQUACULTURE,2003,219(1-4):221-231.
APA Li, FH,Xiang, JH,Zhou, LH,Wu, CG,&Zhang, XJ.(2003).Optimization of triploid induction by heat shock in Chinese shrimp Fenneropenaeus chinensis.AQUACULTURE,219(1-4),221-231.
MLA Li, FH,et al."Optimization of triploid induction by heat shock in Chinese shrimp Fenneropenaeus chinensis".AQUACULTURE 219.1-4(2003):221-231.

入库方式： OAI收割

来源：海洋研究所

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