Cryopreservation of flounder (Paralichthys olivaceus) sperm with a practical methodology
文献类型：期刊论文
| 作者 | Zhang, YZ; Zhang, SC; Liu, XZ; Xu, YY; Wang, CL; Sawant, MS; Li, J ; Chen, SL
|
| 刊名 | THERIOGENOLOGY
 |
| 出版日期 | 2003-09-15 |
| 卷号 | 60期号:5页码:989-996 |
| 关键词 | Flounder Paralichthys Olivaceus Sperm Cryopreservation Glycerol Dimethyl Sulphoxide Methanol |
| ISSN号 | 0093-691X |
| DOI | 10.1016/S0093-691X(03)00097-9 |
| 文献子类 | Article |
| 英文摘要 | A simple and convenient protocol for the cryopreservation of the flounder (Paralichthys olivaceus) sperm was established for "on the spot" cryopreservation of large quantities of semen. The use of three cryoprotectants, dimethyl sulphoxide (DMSO), glycerol (Gly) and methanol was tested in the method. The percentage of motile sperm present in semen after it had been frozen and thawed in the presence of DMSO, Gly or methanol was 60.5 +/- 3.6, 79.17 +/- 4.5 and 13.25 +/- 4.7%, respectively. The fertilization rates of this sperm were 67.06 +/- 15.1, 76.20 +/- 10.0 and 44.93 +/- 22.6%, while the hatching rates of eggs fertilized with this sperm were 37.40 +/- 8.3, 48.18 +/- 25.7 and 23.35 +/- 10.8%, respectively. It was found that Gly and DMSO were better cryoprotectants than methanol, with Gly giving the best overall results. Under scanning electron microscopy, it could be seen that while the majority of the frozen-thawed sperm remained morphologically normal, some exhibited lost or dilated mitochondria, swollen mid-pieces, broken tails, or damaged cell membrane, which probably caused the decrease in motility and fertility of the frozen-thawed sperm. (C) 2003 Elsevier Science Inc. All rights reserved.; A simple and convenient protocol for the cryopreservation of the flounder (Paralichthys olivaceus) sperm was established for "on the spot" cryopreservation of large quantities of semen. The use of three cryoprotectants, dimethyl sulphoxide (DMSO), glycerol (Gly) and methanol was tested in the method. The percentage of motile sperm present in semen after it had been frozen and thawed in the presence of DMSO, Gly or methanol was 60.5 +/- 3.6, 79.17 +/- 4.5 and 13.25 +/- 4.7%, respectively. The fertilization rates of this sperm were 67.06 +/- 15.1, 76.20 +/- 10.0 and 44.93 +/- 22.6%, while the hatching rates of eggs fertilized with this sperm were 37.40 +/- 8.3, 48.18 +/- 25.7 and 23.35 +/- 10.8%, respectively. It was found that Gly and DMSO were better cryoprotectants than methanol, with Gly giving the best overall results. Under scanning electron microscopy, it could be seen that while the majority of the frozen-thawed sperm remained morphologically normal, some exhibited lost or dilated mitochondria, swollen mid-pieces, broken tails, or damaged cell membrane, which probably caused the decrease in motility and fertility of the frozen-thawed sperm. (C) 2003 Elsevier Science Inc. All rights reserved. |
| 学科主题 | Reproductive Biology ; Veterinary Sciences |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000185216600018 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3275]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Ocean Univ Qingdao, Dept Marine Biol, Qingdao 266003, Peoples R China 2.Liaocheng Univ, Dept Life Sci, Liaocheng 252059, Peoples R China 3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, YZ,Zhang, SC,Liu, XZ,et al. Cryopreservation of flounder (Paralichthys olivaceus) sperm with a practical methodology[J]. THERIOGENOLOGY,2003,60(5):989-996. |
| APA | Zhang, YZ.,Zhang, SC.,Liu, XZ.,Xu, YY.,Wang, CL.,...&Chen, SL.(2003).Cryopreservation of flounder (Paralichthys olivaceus) sperm with a practical methodology.THERIOGENOLOGY,60(5),989-996. |
| MLA | Zhang, YZ,et al."Cryopreservation of flounder (Paralichthys olivaceus) sperm with a practical methodology".THERIOGENOLOGY 60.5(2003):989-996. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。