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Effects of cell density, light intensity and mixing on Undaria pinnatifida gametophyte activity in a photobioreactor

文献类型：期刊论文

作者Zou, M; Zhou, BC; Li, BJ; Sun, DH; Zeng, CK
刊名BIOMOLECULAR ENGINEERING
出版日期2003-07-01
卷号20期号:4-6页码:281-284
关键词Gametophytes Light Intensity Cell Density Mixing Undaria Pinnatifida Photosynthetic Activity
ISSN号1389-0344
DOI10.1016/S1389-0344(03)00071-6
文献子类Article
英文摘要An on-line controlled 7 1 sterilizable photobioreactor was used for the optimisation of a culture of gametophytes of Undaria pinnatifida. The gametophytes, which had been stored for three years in a culture cabinet at 16 degreesC, could rapidly grow in the photobioreactor under controlled conditions. The rate of increase of dissolved oxygen and pH were used to monitor the photosynthetic activity. Optimal gametophytes density changed varying the light intensity. The optimal cell densities were 3.24 and 3.45 g FW l(-1) when the cultures were exposed to 61.7 and 82.3 muE m(-2) s(-1), respectively. The optimal cell density was higher under a high photon flux density (PFD) than under low PFD. On the other hand, the optimal light intensities were different for different cell density cultures. The light saturation point was higher at high cell density cultures than at low cell density cultures. The optimal rotational speed was 150 rpm for high cell density culture in the photobioreactor. (C) 2003 Elsevier B.V. All rights reserved.; An on-line controlled 7 1 sterilizable photobioreactor was used for the optimisation of a culture of gametophytes of Undaria pinnatifida. The gametophytes, which had been stored for three years in a culture cabinet at 16 degreesC, could rapidly grow in the photobioreactor under controlled conditions. The rate of increase of dissolved oxygen and pH were used to monitor the photosynthetic activity. Optimal gametophytes density changed varying the light intensity. The optimal cell densities were 3.24 and 3.45 g FW l(-1) when the cultures were exposed to 61.7 and 82.3 muE m(-2) s(-1), respectively. The optimal cell density was higher under a high photon flux density (PFD) than under low PFD. On the other hand, the optimal light intensities were different for different cell density cultures. The light saturation point was higher at high cell density cultures than at low cell density cultures. The optimal rotational speed was 150 rpm for high cell density culture in the photobioreactor. (C) 2003 Elsevier B.V. All rights reserved.
学科主题Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Genetics & Heredity
URL标识查看原文
语种英语
WOS记录号WOS:000184977900023
公开日期2010-12-22
源URL[http://ir.qdio.ac.cn/handle/337002/3277]  
专题海洋研究所_实验海洋生物学重点实验室
作者单位1.Yantai Teachers Coll, Biol Dept, Yantai 264025, Shandong, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
3.Yantai Univ, Ocean Coll, Yantai 264005, Peoples R China
推荐引用方式
GB/T 7714		 Zou, M,Zhou, BC,Li, BJ,et al. Effects of cell density, light intensity and mixing on Undaria pinnatifida gametophyte activity in a photobioreactor[J]. BIOMOLECULAR ENGINEERING,2003,20(4-6):281-284.
APA Zou, M,Zhou, BC,Li, BJ,Sun, DH,&Zeng, CK.(2003).Effects of cell density, light intensity and mixing on Undaria pinnatifida gametophyte activity in a photobioreactor.BIOMOLECULAR ENGINEERING,20(4-6),281-284.
MLA Zou, M,et al."Effects of cell density, light intensity and mixing on Undaria pinnatifida gametophyte activity in a photobioreactor".BIOMOLECULAR ENGINEERING 20.4-6(2003):281-284.

入库方式： OAI收割

来源：海洋研究所

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