The experimental research of R-phycoerythrin subunits on cancer treatment: A new photosensitizer in PDT
文献类型：期刊论文
| 作者 | Huang, B; Wang, GC; Zeng, CK; Li, ZG |
| 刊名 | CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
 |
| 出版日期 | 2002-02-01 |
| 卷号 | 17期号:1页码:35-42 |
| 关键词 | Bilin Attachment Sites Photodynamic Therapy Cell-death B-phycoerythrin Alpha-subunit Beta-subunit Apoptosis Tumors |
| ISSN号 | 1084-9785 |
| 文献子类 | Article |
| 英文摘要 | The mouse tumor cell 5180 and human liver carcinoma cell SMC 7721 cells were first treated with R-PE and its subunits (alpha, beta, gamma subunits), then irradiated with Argon laser (496 nm, 28.8 J/cm(2)). Survival rate was measured by MTT method. In order to compare the phototoxicity in normal cells, the mouse marrow cells were treated with photofrin II and beta-subunit, irradiated with 45 J/cm(2) of light; survival rate was also measured by MTT method. The result showed that R-PE subunits had better PDT effect on s180 cells than R-PE and lower phototoxicity in marrow cells than photofrin II Flow cytometric analysis showed that PDT results in a growth inhibition and a G(0)-G(1) cell cycle arrest in SMC 7721 cells. The tumor cells inhibited by PDT in vivo were morphologically observed by TEM, the tumor cell death was daze to the occlusion of tumor blood vessels and inducement of cell programmed death in nuclei. Therefore, with the advantage in special fluorescence activity, loth molecular weight, good light absorbent character and weak phototoxicity, R-PE subunit is art attractive option for improving the selectivity of PDT.; The mouse tumor cell 5180 and human liver carcinoma cell SMC 7721 cells were first treated with R-PE and its subunits (alpha, beta, gamma subunits), then irradiated with Argon laser (496 nm, 28.8 J/cm(2)). Survival rate was measured by MTT method. In order to compare the phototoxicity in normal cells, the mouse marrow cells were treated with photofrin II and beta-subunit, irradiated with 45 J/cm(2) of light; survival rate was also measured by MTT method. The result showed that R-PE subunits had better PDT effect on s180 cells than R-PE and lower phototoxicity in marrow cells than photofrin II Flow cytometric analysis showed that PDT results in a growth inhibition and a G(0)-G(1) cell cycle arrest in SMC 7721 cells. The tumor cells inhibited by PDT in vivo were morphologically observed by TEM, the tumor cell death was daze to the occlusion of tumor blood vessels and inducement of cell programmed death in nuclei. Therefore, with the advantage in special fluorescence activity, loth molecular weight, good light absorbent character and weak phototoxicity, R-PE subunit is art attractive option for improving the selectivity of PDT. |
| 学科主题 | Oncology ; Medicine, Research & Experimental ; Pharmacology & Pharmacy ; Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| WOS记录号 | WOS:000174233300005 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3281]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Univ Sci & Technol China, Coll Life Sci, Hefei 230026, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, B,Wang, GC,Zeng, CK,et al. The experimental research of R-phycoerythrin subunits on cancer treatment: A new photosensitizer in PDT[J]. CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS,2002,17(1):35-42. |
| APA | Huang, B,Wang, GC,Zeng, CK,&Li, ZG.(2002).The experimental research of R-phycoerythrin subunits on cancer treatment: A new photosensitizer in PDT.CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS,17(1),35-42. |
| MLA | Huang, B,et al."The experimental research of R-phycoerythrin subunits on cancer treatment: A new photosensitizer in PDT".CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS 17.1(2002):35-42. |
入库方式： OAI收割
来源：海洋研究所
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