Expression of hepatitis B surface antigen gene (HBsAg) in Laminaria japonica (Laminariales, Phaeophyta)
文献类型：期刊论文
| 作者 | Jiang, P; Qin, S; Tseng, CK |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2002-09-01 |
| 卷号 | 47期号:17页码:1438-1440 |
| 关键词 | Laminaria Japonica Genetic Engineering Hepatitis b Surface Antigen (Hbsag) Expression System Vaccine |
| ISSN号 | 1001-6538 |
| 文献子类 | Article |
| 英文摘要 | A transformation model for Laminaria japonica was established from 1993 to 1998, on the basis of which the transgenic kelp with heterologous gene encoding hepatitis B surface antigen (HBsAg) was obtained by using the micro-particle bombardment transformation method. Results of quantitative ELISA showed that HBsAg in transgenic kelp was 0.529 mug/mg soluble proteins on average and the highest value was 2.497 mug/mg, implying that recombinant HBsAg had natural epitope. Further support for the integration of HBsAg gene into kelp genome was obtained by PCR-Southern and total DNA hybridization. Prospect of kelp bio-reactor producing high value materials such as edible HBV vaccine was discussed as well.; A transformation model for Laminaria japonica was established from 1993 to 1998, on the basis of which the transgenic kelp with heterologous gene encoding hepatitis B surface antigen (HBsAg) was obtained by using the micro-particle bombardment transformation method. Results of quantitative ELISA showed that HBsAg in transgenic kelp was 0.529 mug/mg soluble proteins on average and the highest value was 2.497 mug/mg, implying that recombinant HBsAg had natural epitope. Further support for the integration of HBsAg gene into kelp genome was obtained by PCR-Southern and total DNA hybridization. Prospect of kelp bio-reactor producing high value materials such as edible HBV vaccine was discussed as well. |
| 学科主题 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000177653300007 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3283]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, P,Qin, S,Tseng, CK. Expression of hepatitis B surface antigen gene (HBsAg) in Laminaria japonica (Laminariales, Phaeophyta)[J]. CHINESE SCIENCE BULLETIN,2002,47(17):1438-1440. |
| APA | Jiang, P,Qin, S,&Tseng, CK.(2002).Expression of hepatitis B surface antigen gene (HBsAg) in Laminaria japonica (Laminariales, Phaeophyta).CHINESE SCIENCE BULLETIN,47(17),1438-1440. |
| MLA | Jiang, P,et al."Expression of hepatitis B surface antigen gene (HBsAg) in Laminaria japonica (Laminariales, Phaeophyta)".CHINESE SCIENCE BULLETIN 47.17(2002):1438-1440. |
入库方式： OAI收割
来源：海洋研究所
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