Analysis of expressed sequence tags of a marine red alga, Gracilaria lemaneiformis
文献类型：期刊论文
| 作者 | Sun, X ; Yang, GP; Mao, YX; Zhang, XC; Sui, ZH; Qin, S
|
| 刊名 | PROGRESS IN NATURAL SCIENCE
 |
| 出版日期 | 2002-07-01 |
| 卷号 | 12期号:7页码:518-523 |
| 关键词 | Gracilaria Lemaneiformis Expressed Sequence Tags (Est) Homologous Sequence |
| ISSN号 | 1002-0071 |
| 文献子类 | Article |
| 英文摘要 | The expressed sequence tags (EST) has been proved to be a useful tool for discovering and identifying functional genes, especially in some species whose genetic information is unavailable. A total of 180 ESTs have been generated from a cDNA library of gametophytic Gracilaria lemaneiformis in this study. These clones are clustered into 151 groups, among which 8 groups are highly homologous to chloroplast genes and are abundant in the library. After searching for matches in the EST database of red alga, 22 groups are found to match with the registered ESTs of Rhadophyta and 6 with Gracilaria. Searching in the protein database reveal that 73 non-redundant clones have significant similarity to some known sequences, the majority of which are involved in photosynthesis, DNA transcription or translation, and 6, 4 and 3 clones are associated with growth or development, signal transduction and stress or defense response, respectively.; The expressed sequence tags (EST) has been proved to be a useful tool for discovering and identifying functional genes, especially in some species whose genetic information is unavailable. A total of 180 ESTs have been generated from a cDNA library of gametophytic Gracilaria lemaneiformis in this study. These clones are clustered into 151 groups, among which 8 groups are highly homologous to chloroplast genes and are abundant in the library. After searching for matches in the EST database of red alga, 22 groups are found to match with the registered ESTs of Rhadophyta and 6 with Gracilaria. Searching in the protein database reveal that 73 non-redundant clones have significant similarity to some known sequences, the majority of which are involved in photosynthesis, DNA transcription or translation, and 6, 4 and 3 clones are associated with growth or development, signal transduction and stress or defense response, respectively. |
| 学科主题 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000176691600006 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3287]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 海洋研究所_海洋生态与环境科学重点实验室 |
| 作者单位 | 1.Ocean Univ Qingdao, Coll Marine Life Sci, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, X,Yang, GP,Mao, YX,et al. Analysis of expressed sequence tags of a marine red alga, Gracilaria lemaneiformis[J]. PROGRESS IN NATURAL SCIENCE,2002,12(7):518-523. |
| APA | Sun, X,Yang, GP,Mao, YX,Zhang, XC,Sui, ZH,&Qin, S.(2002).Analysis of expressed sequence tags of a marine red alga, Gracilaria lemaneiformis.PROGRESS IN NATURAL SCIENCE,12(7),518-523. |
| MLA | Sun, X,et al."Analysis of expressed sequence tags of a marine red alga, Gracilaria lemaneiformis".PROGRESS IN NATURAL SCIENCE 12.7(2002):518-523. |
入库方式： OAI收割
来源：海洋研究所
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