Transient expression of lacZ in bombarded unicellular green alga Haematococcus pluvialis
文献类型：期刊论文
| 作者 | Teng, CY; Qin, S; Liu, JG; Yu, DZ; Liang, CW; Tseng, CK |
| 刊名 | JOURNAL OF APPLIED PHYCOLOGY
 |
| 出版日期 | 2002-12-01 |
| 卷号 | 14期号:6页码:497-500 |
| 关键词 | Green Alga Haematococcus Pluvialis Lacz Micro-particle Bombardment Sv40 Promoter |
| ISSN号 | 0921-8971 |
| 文献子类 | Article |
| 英文摘要 | This paper reports for the first time the transient expression of a reporter gene, LacZ, in the unicellular green alga Haematococcus pluvialis. By employing the micro-particle bombardment method, motile cells in the exponential phase showed transient expression of lacZ. This was detected in bombarded motile cells under the rupture-disc pressures of 3103 KPa and 4137 KPa. Transient expression of LacZ gene could not be observed in non-motile cells of this alga under the same transformation condition. No LacZ background was found in either the motile cells or the non-motile cells. The study suggests a promising potential of the SV40 promoter and the lacZ reporter gene in genetic engineering of unicellular green algae.; This paper reports for the first time the transient expression of a reporter gene, LacZ, in the unicellular green alga Haematococcus pluvialis. By employing the micro-particle bombardment method, motile cells in the exponential phase showed transient expression of lacZ. This was detected in bombarded motile cells under the rupture-disc pressures of 3103 KPa and 4137 KPa. Transient expression of LacZ gene could not be observed in non-motile cells of this alga under the same transformation condition. No LacZ background was found in either the motile cells or the non-motile cells. The study suggests a promising potential of the SV40 promoter and the lacZ reporter gene in genetic engineering of unicellular green algae. |
| 学科主题 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000181010700008 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3289]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Teng, CY,Qin, S,Liu, JG,et al. Transient expression of lacZ in bombarded unicellular green alga Haematococcus pluvialis[J]. JOURNAL OF APPLIED PHYCOLOGY,2002,14(6):497-500. |
| APA | Teng, CY,Qin, S,Liu, JG,Yu, DZ,Liang, CW,&Tseng, CK.(2002).Transient expression of lacZ in bombarded unicellular green alga Haematococcus pluvialis.JOURNAL OF APPLIED PHYCOLOGY,14(6),497-500. |
| MLA | Teng, CY,et al."Transient expression of lacZ in bombarded unicellular green alga Haematococcus pluvialis".JOURNAL OF APPLIED PHYCOLOGY 14.6(2002):497-500. |
入库方式： OAI收割
来源：海洋研究所
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