In vitro assembly of R-phycoerythrin from marine red alga Polysiphonia urceolata
文献类型：期刊论文
| 作者 | Zhang, YZ; Chen, XL; Wang, LS; Zhou, BC; He, JA; Shi, DX; Pang, SJ |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2002 |
| 卷号 | 34期号:1页码:99-103 |
| 关键词 | R-phycoerythrin In Vitro Assembly Scanning Tunneling Microscopy (Stm) |
| ISSN号 | 0582-9879 |
| 文献子类 | Article |
| 英文摘要 | Scanning tunneling microscope was used to investigate the in vitro assembly of R-phycoerythrin (R-PE) from the marine red alga Polysiphonia urceolata. The results showed that R-PE molecules assembled together by disc-to-disc while absorbing on HOPG surface, which just looked like the rods in the phycobilisomes. When the water-soluble R-PE was dissolved in 2% ethanol/water spreading solution, they could form monolayer film at the air/water interface. Similar disc-to-disc array of R-PE was constituted in the two-dimensional Langmuir-Blodgett film by the external force. It could be concluded that, apart from the key role of time linker polypeptides, the in vivo assembly of phycobiliproteins into phycobilisomes is also dependent on the endogenous properties of phycobiliprotein themselves.; Scanning tunneling microscope was used to investigate the in vitro assembly of R-phycoerythrin (R-PE) from the marine red alga Polysiphonia urceolata. The results showed that R-PE molecules assembled together by disc-to-disc while absorbing on HOPG surface, which just looked like the rods in the phycobilisomes. When the water-soluble R-PE was dissolved in 2% ethanol/water spreading solution, they could form monolayer film at the air/water interface. Similar disc-to-disc array of R-PE was constituted in the two-dimensional Langmuir-Blodgett film by the external force. It could be concluded that, apart from the key role of time linker polypeptides, the in vivo assembly of phycobiliproteins into phycobilisomes is also dependent on the endogenous properties of phycobiliprotein themselves. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000173426700019 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3307]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Inst Photog Chem, Beijing 100101, Peoples R China 4.Chinese Acad Sci, Beijing Lab Vacuum Phys, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, YZ,Chen, XL,Wang, LS,et al. In vitro assembly of R-phycoerythrin from marine red alga Polysiphonia urceolata[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2002,34(1):99-103. |
| APA | Zhang, YZ.,Chen, XL.,Wang, LS.,Zhou, BC.,He, JA.,...&Pang, SJ.(2002).In vitro assembly of R-phycoerythrin from marine red alga Polysiphonia urceolata.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,34(1),99-103. |
| MLA | Zhang, YZ,et al."In vitro assembly of R-phycoerythrin from marine red alga Polysiphonia urceolata".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 34.1(2002):99-103. |
入库方式： OAI收割
来源：海洋研究所
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