Foreign gene transfer into Chinese shrimps (Penaeus chinensis) with gene gun
文献类型：期刊论文
| 作者 | Liu, ZY; Xiang, JH ; Zhou, GY; Gong, ZX
|
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2001-05-01 |
| 卷号 | 46期号:9页码:766-770 |
| 关键词 | Gene Gun Gfp Transgenic Shrimps |
| ISSN号 | 1001-6538 |
| 文献子类 | Article |
| 英文摘要 | Plasmids pG DNA-RZ1 with a GFP (green fluorescent protein) reporter gene and a ribozyme gene incising penaeid white spot baculovirus (WSBV) were first introduced into the fertilized eggs of Chinese shrimps by gene gun. The treated and control samples of different development stages were observed with a fluorescent microscope. The transient expression of GFP gene was high in nauplius and zoea larvae. Results from RT-PCR and PCR for adults showed that the foreign genes had been transferred into the shrimps and had expressed the corresponding proteins. This work has established a transgenic method for penaeid shrimps, which will set base for the application of genetic engineering breeding into industry.; Plasmids pG DNA-RZ1 with a GFP (green fluorescent protein) reporter gene and a ribozyme gene incising penaeid white spot baculovirus (WSBV) were first introduced into the fertilized eggs of Chinese shrimps by gene gun. The treated and control samples of different development stages were observed with a fluorescent microscope. The transient expression of GFP gene was high in nauplius and zoea larvae. Results from RT-PCR and PCR for adults showed that the foreign genes had been transferred into the shrimps and had expressed the corresponding proteins. This work has established a transgenic method for penaeid shrimps, which will set base for the application of genetic engineering breeding into industry. |
| 学科主题 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000169054600017 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3315]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Expt Marine Biol Lab, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biochem, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, ZY,Xiang, JH,Zhou, GY,et al. Foreign gene transfer into Chinese shrimps (Penaeus chinensis) with gene gun[J]. CHINESE SCIENCE BULLETIN,2001,46(9):766-770. |
| APA | Liu, ZY,Xiang, JH,Zhou, GY,&Gong, ZX.(2001).Foreign gene transfer into Chinese shrimps (Penaeus chinensis) with gene gun.CHINESE SCIENCE BULLETIN,46(9),766-770. |
| MLA | Liu, ZY,et al."Foreign gene transfer into Chinese shrimps (Penaeus chinensis) with gene gun".CHINESE SCIENCE BULLETIN 46.9(2001):766-770. |
入库方式： OAI收割
来源：海洋研究所
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